Abstract: Objective: To observe the clinical effects of vacuum sealing drainage(VSD) combined with oxygen loaded fluid irrigation in the treatment of chronic wounds.Methods: Sixty-eight chronic wounds patients treated with debridement were randomly divided into the observation group and control group(34 cases each group).The observation group were treated with VSD combined with oxygen loaded fluid irrigation,and the control group were treated with VSD combined with normal saline irrigation.After 7 days of treatment,the tissue fluid oxygen partial pressure of all patients was measured,and the closed negative pressure drainage device was dismantled.After assessing the wound and whole body condition,the patients were treated with phase Ⅱ operation.After 7 days,the bacterial clearance rate,wound granulation tissue coverage,foam dry rate,incidence of drainage blockage,phase Ⅱ operation methods and survival rate of skin graft or skin flap transplantation in two groups were calculated and recorded.Results: The difference of the drainage tube blockage rate between two groups was not statistically significant(P>0.05).The foam dry rate,granulation tissue coverage rate and bacterial clearance rate in observation group were higher than those in control group(P<0.05 to P<0.01).After 7 days of treatment,the local wound tissue oxygen partial pressure in observation group(12.80±4.01) mmHg was significantly higher than that in control group(41.32±4.03) mmHg(P<0.01).The skin graft and skin flap transplantation were used in two groups,the difference of which was not statistically significant(P>0.05).The survival rates of skin graft and skin flap transplantation in observation group were significant higher than those in control group(P<0.01).All patients were followed up for 1 to 3 months.The healing of all skin or skin flap were good,the function and appearance of which was satisfied,and no recurrence was found.Conclusions: The effects of the stage Ⅱ surgery,VSD drainage combined with oxygen loaded fluid irrigating after debriding in the treatment of chronic wounds are good,which is worthy of clinical promotion.