姜黄素逆转卵巢癌对紫杉醇耐药的实验研究

马珊珊; 马玲

doi:10.13898/j.cnki.issn.1000-2200.2016.10.003

姜黄素逆转卵巢癌对紫杉醇耐药的实验研究

马珊珊,
马玲

Experimental study of curcumin reversing drug resistance in ovarian cance

摘要

摘要: 目的：通过观察姜黄素作用人卵巢癌A2780/Taxol细胞株后，逆转该细胞株对紫杉醇的耐药作用，研究卵巢癌细胞对紫杉醇耐药的原因及姜黄素逆转耐药机制。方法：人卵巢癌A2780/Taxol细胞株在体外培养，将细胞分为对照组（A组）和实验组（不同浓度姜黄素组作用细胞）。用四甲基偶氮唑盐微量酶反应比色法检测2组细胞与相同浓度紫杉醇联用后细胞的生长抑制情况；Westernblot方法检测2组细胞内P-糖蛋白（P-gp）和蛋白激酶C-α（PKC-α）的表达情况。结果：四甲基偶氮唑盐微量酶反应比色法得出联合用姜黄素各组细胞生长抑制率均高于A组（P<0.05～P<0.01）；Western blot检测联合用姜黄素各组多药耐药蛋白-1/P-gp和PKC-α表达均较A组显著降低（P<0.01）。结论：姜黄素通过阻碍卵巢细胞中多药耐药蛋白-1/P-gp和PKC-α的表达，增加紫杉醇对细胞的毒性，降低细胞的耐药性。

Abstract: Objective: To observe the reverse effects of curcumin on Taxol resistance to human ovarian cancer A2780/Taxol cells and analyze the mechanism of the reverse effect of curcumin.Methods: Ovarian cancer cell A2780/Taxol were cultured in vitro,the cells were divided into control group(group A) and experimental groups (different concentrations of curcumin),the cell growth inhibition rate combined with the same concentration of paclitaxel in the two groups were detected by by thiazotyl blue tetrazolium bromide(MTT).The protein expressions of P-glycoprotein(P-gp) and Protein Kinase C-α(PKC-α) in the two groups were detected by Westernblot method.Results: Compared with group A,the cell growth inhibition rates were higher in all experimental groups (P<0.05 to P<0.01).The results of Western blot were shown that the expressions of P-gp and PKC-α in experimental group were significantly lower than those in A group(P<0.01).Conclusions: Curcumin blocked the products of multi-drug resistant protein-1/P-gp and PKC-α in ovarian cancer cells,then increased the toxicity of paclitaxel on the cell and reduced the drug resistance.

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