Abstract: Objective: To analyze the pathogenic bacteria characteristics and its drug resistance in neonatal sepsis and ventilator associated pneumonia(VAP) in NICU,and provide the evidence in selecting antibiotics.Methods: The data of blood culture and drug susceptibility in 97 neonatal sepsis patients were retrospectively analyzed.Results: Ninety-seven pathogenic bacteria strains were detected in blood culture.In blood culture,the gram positive bacteria mostly included staphylococcus epidermidis,staphylococcus haemolyticusand staphylococcus hominis,which was highly resistant to penicillin,erythromycin and oxacillin,and individually resistant to linezolid and vancomycin.In blood culture,the gram-negative bacteria mostly included klebsiella pneumoniae and escherichia Coli,which was highly resistant to ampicillin,aztreonam,cefazolin,ceftriaxone and ceftazidime.Forty-two pathogenic bacteria strains were detected in sputum culture(including 1 case with bacterial complicated with fungal infection),In sputum culture,thirty-nine gram negative bacteria were detected,which mainly included acinetobacter bauman,serratia marcescens,stenotrophomonas maltophilia and klebsiella pneumonia,and was multi drug resistant bacteria.The fungi detected by sputum culture was candida albicans.Conclusions: Blood culture result shows that the staphylococcus is main infection in neonatal sepsis,the sputum culture result in ventilator associated pneumonia patient shows that the gram-negative bacilli is main infection.The antibiotics should be reasonably applied according to the test result,which can improve the efficacy and reduce the resistance of bacteria.