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    胡恒贵, 秦淑国, 张长礼, 杜宏斌, 李环如, 黄峰, 韩军. 肽酰基精氨酸脱亚氨酶-4、抗环瓜氨酸肽抗体联合检测在类风湿关节炎诊断中的应用价值[J]. 蚌埠医科大学学报, 2016, 41(10): 1282-1284,1287. DOI: 10.13898/j.cnki.issn.1000-2200.2016.10.007
    引用本文: 胡恒贵, 秦淑国, 张长礼, 杜宏斌, 李环如, 黄峰, 韩军. 肽酰基精氨酸脱亚氨酶-4、抗环瓜氨酸肽抗体联合检测在类风湿关节炎诊断中的应用价值[J]. 蚌埠医科大学学报, 2016, 41(10): 1282-1284,1287. DOI: 10.13898/j.cnki.issn.1000-2200.2016.10.007
    shu
    HU Heng-gui, QIN Shu-guo, ZHANG Chang-li, DU Hong-bin, LI Huan-ru, HUANG Feng, HAN Jun. The application value of the combined detection of peptidylarginine deaminase-4 and anti-cyclic citrullinated peptide antibody in the diagnosis of rheumatoid arthritis[J]. Journal of Bengbu Medical University, 2016, 41(10): 1282-1284,1287. DOI: 10.13898/j.cnki.issn.1000-2200.2016.10.007
    Citation: HU Heng-gui, QIN Shu-guo, ZHANG Chang-li, DU Hong-bin, LI Huan-ru, HUANG Feng, HAN Jun. The application value of the combined detection of peptidylarginine deaminase-4 and anti-cyclic citrullinated peptide antibody in the diagnosis of rheumatoid arthritis[J]. Journal of Bengbu Medical University, 2016, 41(10): 1282-1284,1287. DOI: 10.13898/j.cnki.issn.1000-2200.2016.10.007
    shu

    肽酰基精氨酸脱亚氨酶-4、抗环瓜氨酸肽抗体联合检测在类风湿关节炎诊断中的应用价值

    The application value of the combined detection of peptidylarginine deaminase-4 and anti-cyclic citrullinated peptide antibody in the diagnosis of rheumatoid arthritis

      摘要
    • 摘要: 目的：研究肽酰基精氨酸脱亚胺酶-4（PADI-4）、抗环瓜氨酸抗体（抗CCP抗体）联合检测在类风湿关节炎（RA）诊断中的价值。方法：用酶联免疫吸附试验双抗体夹心法检测58例RA患者（RA组）、40例其他风湿性疾病患者（非RA组）和30名健康志愿者（健康对照组）的血清PADI-4、抗CCP抗体、抗聚角蛋白傲丝蛋白抗体（AFA）、抗突变型瓜氨酸波形蛋白抗体（抗MCV抗体）和抗RA33抗体的浓度，并对其受试者工作特征曲线、灵敏度、特异度、相关性和诊断效能进行分析。结果：RA患者血清中PADI-4、抗CCP抗体、AFA、抗MCV抗体和抗RA33抗体的浓度均显著高于非RA组和健康对照组（P<0.01）；采用受试者工作特征曲线，PADI-4和抗CCP抗体诊断RA的最佳临界值为1.284 U/L和26.365 RU/mL，此时的灵敏度为62.1%和81.1%，特异度为91.4%和95.7%；PADI-4与AFA、抗RA33抗体、抗MCV抗体均呈明显正相关关系（P<0.01）；PADI-4与抗CCP抗体无相关关系（P>0.05）。在多种抗体联合检测中，PADI-4、抗CCP抗体均为阳性诊断RA的特异度高达100.0%，单项检测PADI-4为91.4%，抗CCP抗体为95.7%。结论：PADI-4、抗CCP抗体联合检测能提高RA的特异性和灵敏度，有助于对RA的诊断。

       

      Abstract: Objective: To investigate the value of the combined detection of peptidylarginine deaminase-4(PADI-4) and anti-cyclic citrullinated peptide(anti-CCP) antibody in the diagnosis of rheumatoid arthritis(RA).Methods: The levels of PADI-4,anti-CCP antibody,anti-MCV antibody,AFA antibody and anti-RA33 antibody in 58 patients with RA(RA group),40 patients with other rheumatoid disease(non-RA group) and 30 healthy people(control group) were detected by ELISA.The ROC curve,sensitivity,specificity,relevance and diagnostic effectiveness in all cases were analyzed.Results: The serum levels of PADI-4,anti-CCP antibody,anti-MCV antibody,AFA antibody and anti-RA33 antibody in RA patients were significantly higher than those in non-RA group and control group(P<0.01).The ROC curve showed that the best critical value of PADI-4 and anti-CCP antibody in the diagnosis of RA were 1.284 U/L and 26.365 RU/mL,the sensitivity and specificity of PADI-4 and anti-CCP antibody were 62.1% & 81.1% and 91.4% & 95.7%,respectively.PADI-4 was positive correlation with AFA antibody,anti-RA33 antibody and anti-MCV antibody(P<0.01),but the PADI-4 was not correlation with anti-CCP antibody(P>0.05).Among the combined detection of multiple antibodies,the specificity of the PADI-4 and anti-CCP antibody in the diagnosis of RA was 100.0%,and the positive detection of PADI-4 and anti-CCP antibody were 91.4% and 95.7%,respectively.Conclusions: The combined detection of PADI and anti-CCP antibody can improve the specificity and sensitivity of RA,which can help the diagnosis of RA.

       

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