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    韩正阳, 崔兵, 刘纪君, 贺晓珊, 梅传忠. 索拉非尼联合5-氮杂-2'-脱氧胞苷对肝癌SMMC-7721细胞DNMT3B基因表达的影响[J]. 蚌埠医科大学学报, 2017, 42(3): 285-289. DOI: 10.13898/j.cnki.issn.1000-2200.2017.03.002
    引用本文: 韩正阳, 崔兵, 刘纪君, 贺晓珊, 梅传忠. 索拉非尼联合5-氮杂-2'-脱氧胞苷对肝癌SMMC-7721细胞DNMT3B基因表达的影响[J]. 蚌埠医科大学学报, 2017, 42(3): 285-289. DOI: 10.13898/j.cnki.issn.1000-2200.2017.03.002
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    HAN Zheng-yang, CUI Bing, LIU Ji-jun, HE Xiao-shan, MEI Chuan-zhong. Effect of Sorafenib combined with 5-nitrogen impurity-2'-deoxy cytidine on the expression of DNMT3B gene in hepatocellular carcinoma SMMC-7721 cells[J]. Journal of Bengbu Medical University, 2017, 42(3): 285-289. DOI: 10.13898/j.cnki.issn.1000-2200.2017.03.002
    Citation: HAN Zheng-yang, CUI Bing, LIU Ji-jun, HE Xiao-shan, MEI Chuan-zhong. Effect of Sorafenib combined with 5-nitrogen impurity-2'-deoxy cytidine on the expression of DNMT3B gene in hepatocellular carcinoma SMMC-7721 cells[J]. Journal of Bengbu Medical University, 2017, 42(3): 285-289. DOI: 10.13898/j.cnki.issn.1000-2200.2017.03.002
    shu

    索拉非尼联合5-氮杂-2'-脱氧胞苷对肝癌SMMC-7721细胞DNMT3B基因表达的影响

    Effect of Sorafenib combined with 5-nitrogen impurity-2'-deoxy cytidine on the expression of DNMT3B gene in hepatocellular carcinoma SMMC-7721 cells

      摘要
    • 摘要: 目的：探讨索拉非尼联合5-氮杂-2'-脱氧胞苷（5-Aza-CdR）对人肝癌细胞SMMC-7721的作用及其调控DNMT3B基因表达可能的分子机制。方法：单独及联合给药后以MTT法测定SMMC-7721的增殖，Transwell检测其侵袭，流式细胞仪检测细胞凋亡，Western blot法观察DNMT3B、p-Akt-Ser473及ERK蛋白的表达。结果：索拉非尼、5-Aza-CdR对肝癌SMMC-7721细胞半数抑制浓度（IC50）分别为11、6 μmol/L，联合用药选取（6+4）μmol/L作为后续用药浓度。与对照组比较单药及联合用药均能抑制细胞侵袭、促进细胞凋亡、减少p-Akt-Ser473和DNMT3B蛋白的表达（P<0.05~P<0.01）。结论：索拉非尼和5-Aza-CdR联合用药能有效降低索拉非尼用药浓度，降低肝癌SMMC-7721细胞DNMT3B蛋白表达水平。

       

      Abstract: Objective:To study the effects of the sorafenib combined with 5-nitrogen impurity-2'-deoxy cytidine on human hepatocellular carcinoma SMMC-7721 cells,and investigate the possible molecular mechanism of regulating the DNMT3B gene expression.Methods:After separate and combined medication,the proliferation,invasion and apoptosis of SMMC-7721 cells were detected using MTT method,transwell method and flow cytometry,respectively.The protein expressions of DNMT3B,p-Akt -Ser473 and ERK were measured using Western blotting.Results:The IC50 of sorafenib and 5-nitrogen impurity-2'-deoxy cytidine to hepatocellular carcinoma SMMC-7721 cells were 11 μmol/L and 6 μmol/L respectively.The 6 μmol/L of sorafenib combined with 4 μmol/L of 5-nitrogen impurity-2'-deoxy cytidine was used as the subsequent drug concentration.Compared with the control group,the separate and combined medication could inhibit the cell invasion,promote apoptosis and reduce the protein expressions of p-Akt-Ser473 and DNMT3B(P< 0.05 to P< 0.01).Conclusions:The combined medication of Sorafenib with 5-nitrogen impurity-2'-deoxy cytidine can effectively reduce the sorafenib drug concentration and the protein expression levels of DNMT3B in hepatocellular carcinoma SMMC-7721 cells.

       

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