Abstract: Objective:To investigate the incidence of nosocomial infection in ICU.Methods:The incidence of the nosocomial infection in ICU was investigated using the targeted monitoring method from 2014 to 2015.Results:Eight hundred and fifty-seven patients were monitored.The nosocomial infection in 48 cases were found.The case infection rate,adjustment infection rate and adjustment day infection rate were 5.6%,1.55% and 10.32‰,respectively.The ventilator-associated pneumonia day infection rate,indwelling catheter-associated urinary tract day infection rate and central venous cannula-associated blood flow day infection rate were 16.54‰,1.27‰ and 1.15‰,respectively.Conclusions:The incidence of nosocomial infection in ICU is high,which mainly comes from three kinds of invasive operations.The targeted monitoring can timely obtain the dynamic information of nosocomial infection,and making specific intervention measures can reduce the incidence of hospital infection.