Abstract: Objective: To explore the effects of medical polyethylene surface coated by 2-methacryloyloxy-ethyl phosphoric acid choline(MPC) on the formation of bacterial biofilm.Methods: The surface of medical polyethylene was coated with MPC using ultraviolet light grafting method,and its morphology was observed by scanning electron microscopy.The standard strain of Staphylococcus aureus(ATCC25923) was chose,and the polyethylene plate surface coated by MPC(observation group) and ordinary plate(control group) were co-cultured with 5 mL bacterial liquid(1.5×10⁷ cfu/mL),respectively.After 24 h and 48 h of culturing,the viable bacteria adhered on the surface of plate in two groups were counted,and scanned by electron microscopy.Results: Scanning electron microscopy results showed that the medical polyethylene plate surface coated by MPC was smooth,the original texture of which disappeared.After 24 h and 48 h of culturing,the quantity of viable bacteria in observation group was significantly less than that in control group(P<0.05),and scanning electron microscopy results showed that the amount of adhesion bacterial of plate surface in observation group was significantly less than that in control group(P<0.05).Conclusions: MPC coating on medical polyethylene surface can effectively inhibit the bacterial adhesion,and prevent the bacterial biofilm formation.