Abstract: Objective: To explore the feasibility of direct two-dimensional(D2D) measurement in evaluating the myocardial strain and cardiac function.Methods: The systolic longitudinal strain(LS) and circumferential strain(CS) in 60 normal people were measured using the D2D and STI,respectively.The result between STI and D2D was compared.Results: The absolute value of LS and CS detected by STI and D2D gradually increased and decreased from the basal segment to the apical segment,respectively.The absolute value of LS and CS detected by STI and D2D were positive correlation with LS-LVEF and CS-LVEF.The correlation coefficients of LS-LVEF and CS-LVEF detected by STI were 0.693 and 0.561,respectively,the correlation coefficients of LS-LVEF and CS-LVEF detected by D2D were 0.589 and 0.457,respectively,and the differences of the correlation coefficients of LS-LVEF and CS-LVEF detected by two methods were not statistically significant(P>0.05).D2D could not separate the CS of ventricular walls,which could indirectly accounted by measuring the circumference changes of systolic diastolic heart inner and outer membrane.The absolute value of the measurement results of D2D was higher than that of STI.The LS detected by D2D in infereior wall,posterior septum,anterior septum,posterior wall,anterior wall and laterior wall gradually decreased,and the differences of whose were not statistically significant(P>0.05).The LS detected by STI in infereior wall,laterior wall,anterior septum,posterior wall,posterior septum and anterior wall gradually decreased,and the differences of whose were not statistically significant(P>0.05).The results of STI and D2D methods showed that the endocardial myocardial layers CS was higher than that in the epicardial layer(P<0.05),and the level of mitral valve was significantly higher than that of papillary muscle and apical segment(P<0.05).The absolute value of D2D method was higher than that of STI method (P<0.05),but there was not significantly different in LS-LVEF and CS-LVEF between STI and D2D methods,which indicated that the D2D method instead of STI method was feasible in detecting myocardial strain,and the results of STI and D2D methods were close.Conclusions: The correlation of D2D results with result of STI method is high.The STI method has certain feasibility and accuracy,which is suitable for primary hospital with ordinary equipment.