FMNL2、RAC1在结直肠癌组织中的表达及意义

郭婉薇; 杨海云; 李博; 黎丽旋; 许鸣

doi:10.13898/j.cnki.issn.1000-2200.2017.10.015

FMNL2、RAC1在结直肠癌组织中的表达及意义

Expression and significance of FMNL2 and RAC1 in colorectal cancer

摘要

摘要: 目的：检测FMNL2及RAC1在结直肠癌组织及相应癌旁组织中的表达情况及与临床病理学参数之间的相关性。方法：采用RT-qPCR及免疫组织化学SABC法，分别检测FMNL2及RAC1 mRNA及蛋白在结直肠癌及癌旁组织中的表达。结果：结直肠癌中FMNL2及RAC1 mRNA表达均高于相对应的癌旁组织（P<0.01）；FMNL2和RAC1蛋白表达在结直肠癌组织中的阳性率分别为61.7%、68.3%，高于对应的癌旁组织中20.8%和31.7%（P<0.01）。FMNL2及RAC1蛋白表达均与结直肠癌细胞淋巴结转移、远处转移及TNM分期密切相关（P<0.05~P<0.01），而与病人年龄、性别、肿瘤大小、分化及浸润深度无明显相关关系（P>0.05）。FMNL2的表达与RAC1的表达呈正相关关系（r=0.495，P<0.05）。结论：FMNL2与RAC1在结直肠癌组织中高表达，且与肿瘤恶性生物学行为特别是转移密切相关，联合检测二者的表达对预测肿瘤转移、判断预后具有重要意义。

Abstract: Objective:To explore the expressions of FMNL2 and RAC1 in colorectal cancer(CRC) tissues and corresponding precancerous lesions,and correlation of the expressions with clinical pathological parameters.Methods:The mRNA and protein expressions of FMNL2 and RAC1 in CRC tissues and corresponding precancerous lesions were detected by real-time PCR and immunohistochemistry(IHC) method.Results:The mRNA expressions of FMNL2 and RAC1 in CRC tissues were higher than those in corresponding precancerous lesions(P<0.01).The positive rates of FMNL2 and RAC1 in CRC tissues(61.7% and 68.3%) were higher than those in corresponding precancerous lesions(20.8% and 31.7%),respectively(P<0.05).The expressions of FMNL2 and RAC1 were closely related to the tumor metastasis,lymph node metastasis and TNM staging(P<0.05 to P<0.01),but which was not related to the gender and age of patients,and size,differentiation and invasion of tumor(P>0.05).The expression of FMNL2 was positively correlated with RAC1(r=0.495,P<0.05).Conclusions:The expressions of FMNL2 and RAC1 in CRC tissues are high,which is closely related to the tumor metastasis.The combined detection of FMNL2 and RAC1 have important significances in predicting tumor metastasis and prognosis of tumor.

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