金黄色葡萄球菌L型对宫颈癌细胞生长的影响

胡小冬; 崔洁; 唐素兰; 管俊昌

doi:10.13898/j.cnki.issn.1000-2200.2017.11.002

金黄色葡萄球菌L型对宫颈癌细胞生长的影响

Effect of staphylococcus aureus L-form on the growth of cervical cancer cells

摘要

摘要: 目的：研究金黄色葡萄球菌（金葡菌）L型对体外培养的单层人宫颈癌细胞（HeLa）生长的影响作用。方法：用常规方法诱导金葡菌为L型后感染HeLa细胞，采用光学显微镜、透射电子显微镜检测金葡菌L型黏附及侵入细胞情况，并采用改良的MTT比色法检测细胞增殖能力。结果：光镜、电镜检查显示金葡菌L型能够黏附并且进入HeLa细胞；改良的MTT法检测表明金葡菌L型能够促进HeLa细胞增殖（P<0.01）。结论：金葡菌L型能够感染并促进HeLa细胞增殖生长，揭示金葡菌L型可能与宫颈癌的发生有关。

Abstract: Objective:To observe the effects of Staphylococcus aureus L-form on the growth of human cervical cancer cells(HeLa) in vitro.Methods:The HeLa cells were infected with Staphylococcus aureus L-form by routine method.The adhesion and invasion of Staphylococcus aureus L-form were detected using light and electron microscopy,and the proliferation of HeLa cells was detected using improved MTT assay.Results:The results of light and electron microscopy L-form showed that the Staphylococcus aureus L-form could adhere and invade into HeLa cells.The result of improved MTT method showed that Staphylococcus aureus L-form could promote the proliferation of HeLa cells.Conclusions:Staphylococcus aureus L-form can infect and promote the growth of HeLa cells,which reveals that Staphylococcus aureus L-form may be one of inducting factor of cervical cancer.

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