Abstract: Objective:To establish an HPLC method for the content determination of gambogenic acid in tumor cells.Methods:The gambogenic acid in cells was measured using HPLC.The chromatographic column was COSMOSIL C₁₈ column,the mobile phase was 90% methanol:0.1% aqueous phosphoric acid(90:10,V:V),the flow rate was 1.0 mL/min,the column temperature was 30℃,the detection wavelength was 360 nm,and the injection volume was 20 μL.Results:The linear relationship between the 0.05 to 30 μg/mL of gambogenic acid and peak area was good(P<0.01).The precision and stability of RSD was lower than 10%.The accuracy of the measure value was less than 10%,the specificity was good.The repeatability of sample determination at the same time and concentration of gambogenic acid was good.Conclusions:The method is simple,convenient and accurate,which is suit for the determination of gambogenic acid in tumor cells.