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    黄坤玲, 刘建华, 刘向飞, 帅金凤, 牛波, 路素坤, 曹丽洁. 外周血Th17/Treg及相关细胞因子表达在喘息婴幼儿发病中的作用及意义[J]. 蚌埠医科大学学报, 2017, 42(11): 1513-1516. DOI: 10.13898/j.cnki.issn.1000-2200.2017.11.024
    引用本文: 黄坤玲, 刘建华, 刘向飞, 帅金凤, 牛波, 路素坤, 曹丽洁. 外周血Th17/Treg及相关细胞因子表达在喘息婴幼儿发病中的作用及意义[J]. 蚌埠医科大学学报, 2017, 42(11): 1513-1516. DOI: 10.13898/j.cnki.issn.1000-2200.2017.11.024
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    HUANG Kun-ling, LIU Jian-hua, LIU Xiang-fei, SHUAI Jin-feng, NIU Bo, LU Su-kun, CAO Li-jie. The significance of Th17/Treg and related cytokine levels of peripheral blood in infants with wheezing[J]. Journal of Bengbu Medical University, 2017, 42(11): 1513-1516. DOI: 10.13898/j.cnki.issn.1000-2200.2017.11.024
    Citation: HUANG Kun-ling, LIU Jian-hua, LIU Xiang-fei, SHUAI Jin-feng, NIU Bo, LU Su-kun, CAO Li-jie. The significance of Th17/Treg and related cytokine levels of peripheral blood in infants with wheezing[J]. Journal of Bengbu Medical University, 2017, 42(11): 1513-1516. DOI: 10.13898/j.cnki.issn.1000-2200.2017.11.024
    shu

    外周血Th17/Treg及相关细胞因子表达在喘息婴幼儿发病中的作用及意义

    The significance of Th17/Treg and related cytokine levels of peripheral blood in infants with wheezing

      摘要
    • 摘要: 目的：探讨外周血Th17/Treg及相关细胞因子表达在喘息婴幼儿发病中的作用及意义。方法：选取急性发作期喘息婴幼儿60例（喘息组）及下呼吸道感染但不伴喘息发作的婴幼儿50例（非喘息组），同时选取同期体检健康婴幼儿50名（对照组），并根据患儿既往喘息发作次数，将喘息组分为A1组（首次喘息组，34例）及A2组（反复喘息组，喘息发作≥ 2次，26例）；根据有无合并哮喘高危因素分为B1组（合并高危因素组，25例）和B2组（未合并高危因素组，35例），分别采用流式细胞仪检测各组婴幼儿Th17细胞、Treg细胞在外周血中的比例，酶联免疫吸附法测定各组婴幼儿血清中的白细胞介素-17（IL-17）、IL-10的表达。结果：3组外周血单个核细胞Th17、Treg细胞百分数及Treg/Th17比较差异均有统计学意义（P<0.01）；其中喘息组、非喘息组的Treg细胞及Treg/Th17百分数均显著低于对照组（P<0.01），而Th17细胞百分数显著高于对照组（P<0.01）；且喘息组Treg细胞及Treg/Th17百分数显著低于非喘息组（P<0.01），Th17细胞百分数显著高于非喘息组（P<0.01）。3组血清中IL-17、IL-10水平比较差异均有统计学意义（P<0.01），其中喘息组、非喘息组的IL-17显著高于对照组（P<0.01），IL-10水平显著低于对照组（P<0.01），喘息组的IL-17显著高于非喘息组（P<0.01），IL-10水平显著低于非喘息组（P<0.01）。A1组外周血Th17百分数高于A2组（P<0.05），Treg细胞及Treg/Th17百分数显著低于A2组（P<0.01）；A1组IL-17显著高于A2组（P<0.01），IL-10显著低于A2组（P<0.01）。B1组外周血Th17百分数显著高于B2组（P<0.01），Treg细胞Treg/Th17百分数显著低于B2组（P<0.01）；B1组IL-17显著高于B2组（P<0.01），IL-10显著低于B2组（P<0.01）。结论：早期监测外周血Th17/Treg及相关细胞因子表达可为有支气管哮喘发展倾向的患儿提供早期诊断及早期干预的依据。

       

      Abstract: Objective:To investigate the role and significance of Th17/Treg and its related cytokine levels in the pathogenesis of wheezing in infants.Methods:Sixty acute attack wheezing infants,50 lower respiratory infection infants without wheezing and 50 healthy infants were divided into the wheezing group,nonwheezing group and control group,respectively.According to the number of wheezing,the wheezing group was subdivided into the group A1(first wheezing group,34 cases) and group A2(repeat wheezing group,wheezing number ≥ 2 times,26 cases).According to the risk factor of wheezing,the wheezing group was subdivided into the group B1(complicated with high risk factor group,25 cases) and group B2(complicated without high risk factor group,35 cases).The proportions of Th17 and Treg cells in peripheral blood in all groups were detected using flow cytometry,and the serum levels of interleukin-17(IL-17) and interleukin-10(IL-10) in all group were detected using ELISA.Results:The differences of the percentages of Th17 and Treg cells,and Treg/Th17 between three groups were statistically significant(P<0.01).The Treg cells and percentage of Treg/Th17 in wheezing group and nonwheezing group were significantly lower than those in control group(P<0.01),and the percentage of Th17 cells in wheezing group and nonwheezing group were significantly higher than that in control group(P<0.01).The Treg cells and percentage of Treg/Th17 in wheezing group were significantly lower than those in nonwheezing group(P<0.01),and the percentage of Th17 cells in wheezing group was significantly higher than that in nonwheezing group(P<0.01).The differences of the serum levels of IL-17 and IL-10 between three groups were statistically significant(P<0.01).The serum levels of IL-17 in wheezing group and nonwheezing group were significantly higher than that in control group(P<0.01),and the serum levels of IL-10 in wheezing group and nonwheezing group were significantly lower than that in control group(P<0.01).The serum level of IL-17 in wheezing group was significantly higher than that in nonwheezing group(P<0.01),and the serum level of IL-10 in wheezing group was significantly lower than that in nonwheezing group(P<0.01).The percentage of Th17 cells in group A1 was higher than that in group A2(P<0.01),the Treg cells and percentage of Treg/Th17 in group A1 were significantly lower than those in group A2(P<0.01).The IL-17 level in group A1 was significantly higher than that in group A2(P<0.01),and the IL-10 level in group A1 was significantly lower than that in group A2(P<0.01).The percentage of Th17 cells in group B1 was significantly higher than that in group B2(P<0.01),and the Treg cells and percentage of Treg/Th17 in group B1 were significantly lower than those in group B2(P<0.01).The IL-17 level in group B1 was significantly higher than that in group B2(P<0.01),and the IL-10 level in group B1 was significantly lower than that in group B2(P<0.01).Conclusions:Early detection of Th17/Treg and related cytokines in peripheral blood may provide the basis of early diagnosis and intervention for children with bronchial asthma development tendency.

       

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