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    于嘉伟, 周艳, 马利林, 钱飞, 孙乃至, 肖建佳, 殷威, 朱建伟. miRNA-101在胃癌组织和细胞中的表达及对胃癌生物学行为的影响[J]. 蚌埠医科大学学报, 2017, 42(12): 1589-1592. DOI: 10.13898/j.cnki.issn.1000-2200.2017.12.005
    引用本文: 于嘉伟, 周艳, 马利林, 钱飞, 孙乃至, 肖建佳, 殷威, 朱建伟. miRNA-101在胃癌组织和细胞中的表达及对胃癌生物学行为的影响[J]. 蚌埠医科大学学报, 2017, 42(12): 1589-1592. DOI: 10.13898/j.cnki.issn.1000-2200.2017.12.005
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    YU Jia-wei, ZHOU Yan, MA Li-lin, QIAN Fei, SUN Nai-zhi, XIAO Jian-jia, YIN Wei, ZHU Jian-wei. The expression level of miRNA-101 in gastric cancer tissue and cell,and its influence on biological behavior of gastric cancer[J]. Journal of Bengbu Medical University, 2017, 42(12): 1589-1592. DOI: 10.13898/j.cnki.issn.1000-2200.2017.12.005
    Citation: YU Jia-wei, ZHOU Yan, MA Li-lin, QIAN Fei, SUN Nai-zhi, XIAO Jian-jia, YIN Wei, ZHU Jian-wei. The expression level of miRNA-101 in gastric cancer tissue and cell,and its influence on biological behavior of gastric cancer[J]. Journal of Bengbu Medical University, 2017, 42(12): 1589-1592. DOI: 10.13898/j.cnki.issn.1000-2200.2017.12.005
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    miRNA-101在胃癌组织和细胞中的表达及对胃癌生物学行为的影响

    The expression level of miRNA-101 in gastric cancer tissue and cell,and its influence on biological behavior of gastric cancer

      摘要
    • 摘要: 目的：探讨miRNA-101在胃癌组织和细胞中的表达情况及对胃癌细胞生物学行为的影响。方法：应用实时荧光定量PCR法分析31例胃癌组织和癌旁组织样本以及人胃癌细胞系SGC-7901、MKN45、人正常胃黏膜上皮细胞系GES1中miRNA-101的表达水平；建立miRNA-101重组腺病毒载体感染胃癌细胞系SGC-7901、MKN45，对照组为感染阴性对照病毒的胃癌细胞系SGC-7901、MKN45，MTT法检测miRNA-101对胃癌细胞增殖能力的影响；Transwell小室法检测胃癌细胞的迁移和侵袭能力。结果：胃癌组织中miR-101表达水平明显低于癌旁组织（P<0.01）；miR-101在细胞系SGC-7901和MKN45中的表达量明显低于人正常胃黏膜上皮细胞系GES1（P<0.01）。孵育48 h后MKN45细胞miRNA-101组的细胞增殖能力低于其对照组（P<0.05）；而孵育72 h后SGC-7901细胞和MKN45细胞的miRNA-101组细胞增殖能力均亦低于相应对照组（P<0.05）。迁移试验结果显示，miRNA-101组迁移的胃癌细胞SGC-7901和MKN45的平均细胞数明显低于相应对照组（P<0.01）；侵袭实验结果显示，miRNA-101组侵袭的胃癌细胞SGC-7901和MKN45的平均细胞数亦明显低于其对照组（P<0.01）。结论：miRNA-101在胃癌组织和胃癌细胞系中表达下调，转染miRNA-101可明显降低SGC-7901和MKN45细胞的增殖、迁移、侵袭能力，miRNA-101可能参与了胃癌疾病的发生及发展。

       

      Abstract: Objective:To explore the expression levels of miRNA-101 in gastric cancer tissue and cell,and its influence on biological behavior of gastric cancer cells.Methods:The expression levels of miRNA-101 in 31 gastric carcinoma tissue and paraneoplastic tissue samples,human gastric carcinoma cell line SGC-7901 and MKN45,and normal human gastric epithelial cell line GES1 were detected using real-time PCR method.The recombinant adenovirus vector containing miRNA-101 was established,and transfected into the gastric cancer cell line SGC-7901 and MKN45.The effect of miRNA-101 on gastric cancer cell proliferation was detected by MTT method,and the migration and invasion of gastric cancer cells were detected by Transwell method.Results:The expression level of miRNA-101 in gastric cancer tissue was significantly lower than that in adjacent tissue(P<0.01).The expression levels of miRNA-101 in SGC-7901 and MKN45 cell lines were significantly lower than that in GES1 cells(P<0.01).The proliferation of MKN45 cells in miRNA-101 group after 48 h of incubation was significantly lower than that in control group(P<0.05).The proliferations of SGC-7901 and MKN45 cells in miRNA-101 group after 72 h of incubation were lower than that in control group(P<0.05).The migration test results showed that the average migrated number of SGC-7901 and MKN45 cells in miRNA-101 group were significantly lower than that in control group(P<0.01).The invasion test results showed that the average invaded number of SGC-7901 and MKN45 cells in miRNA-101 group were significantly lower than that in control group(P<0.01).Conclusions:The expression levels of miRNA-101 in gastric cancer tissue and gastric cancer cell lines downregulate,the transfection of miRNA-101 can significantly decrease the abilities of proliferation,migration and invasion of SGC-7901 and MKN45 cells.The miRNA-101 may be involved in the occurrence and development of gastric diseases.

       

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