高级检索
    陈辰, 王双燕, 夏玉军. Kif15调控大鼠星形胶质细胞增殖、细胞周期及凋亡的研究[J]. 蚌埠医科大学学报, 2018, 43(1): 8-12. DOI: 10.13898/j.cnki.issn.1000-2200.2018.01.003
    引用本文: 陈辰, 王双燕, 夏玉军. Kif15调控大鼠星形胶质细胞增殖、细胞周期及凋亡的研究[J]. 蚌埠医科大学学报, 2018, 43(1): 8-12. DOI: 10.13898/j.cnki.issn.1000-2200.2018.01.003
    shu
    CHEN Chen, WANG Shuang-yan, XIA Yu-jun. Effect of Kif15 on the proliferation,cell cycle and apoptosis of astrocytes in rat[J]. Journal of Bengbu Medical University, 2018, 43(1): 8-12. DOI: 10.13898/j.cnki.issn.1000-2200.2018.01.003
    Citation: CHEN Chen, WANG Shuang-yan, XIA Yu-jun. Effect of Kif15 on the proliferation,cell cycle and apoptosis of astrocytes in rat[J]. Journal of Bengbu Medical University, 2018, 43(1): 8-12. DOI: 10.13898/j.cnki.issn.1000-2200.2018.01.003
    shu

    Kif15调控大鼠星形胶质细胞增殖、细胞周期及凋亡的研究

    Effect of Kif15 on the proliferation,cell cycle and apoptosis of astrocytes in rat

      摘要
    • 摘要: 目的：探讨Kif15调控星形胶质细胞增殖、细胞周期及凋亡的影响及机制。方法：从出生1 d的大鼠分离出星形胶质细胞，分别用NC-小干扰RANA（siRNA）（NC-siRNA组）、Kif15-siRNA转染细胞（Kif15-siRNA组），不做任何处理的细胞作为空白对照组（Control组），48 h后收集细胞，Western blotting检测各组细胞中Kif15的蛋白表达，CCK-8法检测细胞增殖；流式细胞仪检测细胞周期及细胞凋亡率；Western blotting检测增殖细胞核抗原（PCNA）、细胞周期素D1（cyclinD1）、Cleaved caspase3、p38、p-p38蛋白表达情况。结果：转染Kif15-siRNA能显著抑制Kif15的蛋白表达；与对照组及NC-siRNA组比较，Kif15-siRNA组细胞存活率、G2/M期细胞及PCNA、cyclinD1、p-p38蛋白表达显著降低，细胞凋亡率、G1期细胞及Cleaved caspase3蛋白表达显著升高（P<0.01），p38蛋白表达在各组细胞间差异无统计学意义（P>0.05）。结论：抑制星形胶质细胞中Kif15的表达，可显著降低细胞的增殖，阻滞细胞于G1期，促进细胞凋亡，其机制与抑制p38信号通路有关。

       

      Abstract: Objective:To investigate the effects of Kif15 on the proliferation,cell cycle and apoptosis of astrocytes in rat,and its mechanism.Methods:Astrocytes was isolated from one day old rat,the NC-siRNA and Kif15-siRNA were transfected into the cells,and the cells without any treatment was set as the control group.The expression levels of Kif15 protein in each group were detected using Western blotting after 48 h of transfection.The cell proliferation,cell cycle and apoptosis,and protein expression levels of PCNA,cyclinD1,Cleaved caspase3,p38 and p-p38 were detected using CCK8 assay,flow cytometry and Western blotting,respectively.Results:The expression level of Kif15 protein could be significantly inhibited in the cells transfected with Kif15-siRNA.Compared with the control group and NC-siRNA group,the cell survival rate,G2/M cells,and expression levels of PCNA,cyclinD1 and p-p38 proteins significantly decreased,the cell apoptosis rate,G1 cells and expression level of cleaved Caspase 3 protein significantly increased in Kif15-siRNA group(P<0.01),and the difference of the expression level of p38 between three groups was not statistically significant(P>0.05).Conclusions:The inhibiting of Kif15 in astrocytes can significantly reduce the proliferation of cells,block the cell in G1 phase and promote apoptosis,the mechanism of which is related to the inhibition of p38 signaling pathway.

       

      • HTML全文
      • 参考文献(20)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回