Abstract: Objective:To investigate the status of blood-borne occupational exposure and protection,and explore the measures to reduce the blood-borne occupational exposure of medical staff in operation room.Methods:Twenty-four medical staff with blood-borne occupational exposure in operation room were investigated,and the population distribution,generant step,exposure source and handle situation after exposure were analyzed.Results:Doctor was the main medical staff with blood-borne occupational exposure(70.83%),the ratios of blood-borne occupational exposure of medical staff with the age less than forty years,working year less than three years and more than or equal to nine years,and primary title were 79.17%,37.50%,33.33% and 54.17%,respectively.Hand was the main exposure part(91.67%),the thumb and middle fingers were more in the left hand,and the forefinger was more in right hand.The main way of blood-borne occupational exposure was sharp instrument injury(91.67%),and the ratios of the division,transfer and operation steps of surgical instrument during operation,and operation suture step were 45.83% and 37.50%,respectively.The HBV was the main pathogen of exposure(83.33%).All medical staff with blood-borne occupational exposure were immediately treated after exposure in local,the correct rate of local treatment was 83.33%.Eighty medical staff were treated with preventive medication after exposure(75.00%),the main treatment way was the HBIG combined with hepatitis B vaccine,and second HBIG therapy.Conclusions:Definiting the high risk population,main step and sharp instrument of blood-borne occupational exposure in operation room,standardizing operating procedure,necessary protective equipment and right treatment after exposure can reduce the risk of blood-borne occupational exposure and infection after exposure.