刘扬, 李竹琴, 张志刚. 去甲肾上腺素及血管紧张素Ⅱ对大鼠心肌细胞胰岛素受体及胰岛素受体底物1酪氨酸磷酸化的综合效应[J]. 蚌埠医学院学报, 2018, 43(2): 146-149. DOI: 10.13898/j.cnki.issn.1000-2200.2018.02.002
    引用本文: 刘扬, 李竹琴, 张志刚. 去甲肾上腺素及血管紧张素Ⅱ对大鼠心肌细胞胰岛素受体及胰岛素受体底物1酪氨酸磷酸化的综合效应[J]. 蚌埠医学院学报, 2018, 43(2): 146-149. DOI: 10.13898/j.cnki.issn.1000-2200.2018.02.002
    LIU Yang, LI Zhu-qin, ZHANG Zhi-gang. Comprehensive effect of norepinephrine and angiotensin Ⅱ on the tyrosine phosphorylation of insulin receptor and insulin receptor substance 1 in rat cardiac cells[J]. Journal of Bengbu Medical College, 2018, 43(2): 146-149. DOI: 10.13898/j.cnki.issn.1000-2200.2018.02.002
    Citation: LIU Yang, LI Zhu-qin, ZHANG Zhi-gang. Comprehensive effect of norepinephrine and angiotensin Ⅱ on the tyrosine phosphorylation of insulin receptor and insulin receptor substance 1 in rat cardiac cells[J]. Journal of Bengbu Medical College, 2018, 43(2): 146-149. DOI: 10.13898/j.cnki.issn.1000-2200.2018.02.002

    去甲肾上腺素及血管紧张素Ⅱ对大鼠心肌细胞胰岛素受体及胰岛素受体底物1酪氨酸磷酸化的综合效应

    Comprehensive effect of norepinephrine and angiotensin Ⅱ on the tyrosine phosphorylation of insulin receptor and insulin receptor substance 1 in rat cardiac cells

    • 摘要: 目的:初步探讨去甲肾上腺素(NE)与血管紧张素Ⅱ(AngⅡ)对大鼠心肌细胞胰岛素受体β亚基(Ins-Rβ)及胰岛素受体底物1(IRS-1)酪氨酸磷酸化的综合效应,以及心肌胰岛素抵抗的机制。方法:培养新生大鼠心肌细胞。按照浓度梯度,将相同浓度的NE与AngⅡ的混合液加入培养基中,培养72 h后,采用Western blotting法检测胰岛素信号分子蛋白表达水平。另于每个实验组中加入AngⅡ受体阻断剂氯沙坦,分别观察NE与AngⅡ的独立效应。结果:相同浓度的NE与AngⅡ混合液可引起心肌细胞Ins-R、IRS-1及葡萄糖转运体4(GLUT4)等蛋白表达减少,Ins-Rβ亚基酪氨酸磷酸化等蛋白表达减少,IRS-1酪氨酸磷酸化蛋白表达增加(P<0.05)。1 μmol/L氯沙坦对Ins-Rβ亚基酪氨酸磷酸化蛋白表达无显著影响(P>0.05),可减少IRS-1酪氨酸磷酸化蛋白表达(P<0.05)。结论:相同浓度的NE与AngⅡ的共同作用可降低大鼠心肌细胞Ins-Rβ酪氨酸磷酸化水平,升高IRS-1酪氨酸磷酸化水平。NE为引起Ins-Rβ酪氨酸磷酸化水平降低的主要原因,AngⅡ升高为IRS-1酪氨酸磷酸化水平升高的主要原因。

       

      Abstract: Objective: To investigate the comprehensive effects of norepinephrine(NE) and angiotensin Ⅱ(AngⅡ) on tyrosine phosphorylation of insulin receptor β(Ins-Rβ) subunit and insulin receptor substance 1(IRS-1) in rat cardiac cells,and the mechanism of insulin resistance.Methods: The myocardial cells from the neonatal rat(1 to 3d) were primary cultured,and co-cultured with the same concentration of the mixture of NE and AngⅡ for 72 h according to the concentration gradient.The protein expressions of insulin signal molecules were detected using Western blotting.Losartan,Ang Ⅱ receptor blockers,was added into each experiment group,and the independent effect of NE and AngⅡ was observed.Results: Under the same concentration of the mixture of NE and Ang Ⅱ,the protein expression levels of Ins-R,IRS-1 and glucose transporter type 4(GLUT4),and level of tyrosine phosphorylation of Ins-Rβ decreased,and the level of tyrosine phosphorylation of IRS-1 increased in myocardial cells(P<0.05).The effect of 1 μmol/L of losartan on the level of tyrosine phosphorylation of Ins-Rβ was not obvious(P>0.05),which could decrease the level of tyrosine phosphorylation of IRS-1(P<0.05).Conclusions: The same concentration of the mixture of NE and AngⅡ can decrease the tyrosine phosphorylation level of Ins-R β subunit,and increase the tyrosine phosphorylation level of IRS-1.NE is the crucial factor in decreasing the tyrosine phosphorylation level of Ins-Rβ,and the AngⅡ is the crucial factor in increasing the tyrosine phosphorylation level of IRS-1.

       

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