孕鼠给予葡萄球菌肠毒素B对新生子代鼠脾脏淋巴细胞体外增殖的影响

陈杰; 李慧慧; Mohsin Raza kashif; 高淑娴; 周平; 郑庆委; 韦莉; 申林; 管俊昌

doi:10.13898/j.cnki.issn.1000-2200.2018.08.001

孕鼠给予葡萄球菌肠毒素B对新生子代鼠脾脏淋巴细胞体外增殖的影响

Effect of maternal SEB administration on proliferation of splenic lymphocytes of the newborn offspring rats in vitro

摘要

摘要: 目的：观察妊娠期孕鼠给予葡萄球菌肠毒素B（SEB）对其新生子代鼠脾脏淋巴细胞体外增殖的影响。方法：在SD大鼠妊娠第16天给予尾静脉注射15 μg SEB，同时设立磷酸盐缓冲液（PBS）对照组。获取出生后3d的新生鼠脾脏并分离淋巴细胞，将淋巴细胞与ConA或SEB共培养3 d，流式细胞仪检测新生鼠脾脏淋巴细胞亚群，液体闪烁仪测定细胞的增殖能力。结果：SEB组新生鼠脾脏中CD4⁺T细胞比例在ConA或SEB刺激第1天分别较PBS组明显增加，但在第2~3天却明显降低（P<0.05~P<0.01）；而SEB组新生鼠脾脏中CD4⁺TCR Vβ8.2⁺T细胞比例在ConA或SEB刺激的第1天及CD8⁺ TCR Vβ8.2⁺ T细胞比例分别较PBS组明显增加（P<0.05~P<0.01）。SEB组新生鼠脾脏淋巴细胞在ConA或SEB刺激后第1天增殖能力明显强于PBS组，但在第2~3天的增殖能力却又明显低于PBS组（P<0.05~P<0.01）。结论：妊娠期孕鼠给予SEB可以影响新生鼠脾脏淋巴细胞的体外增殖。

Abstract: Objective:To investigate the effects of maternal staphylococcal enterotoxin B(SEB) administration on proliferation of splenic lymphocytes of the newborn offspring rats in vitro.Methods:Pregnant rats on gestational day 16 were injected i.v.15 μg SEB,and the control group was injected using the same volume of PBS.The spleens of newborn offspring rats on the third day after delivery were acquired,and the splenic lymphocytes were separated.The splenic lymphocytes were co-cultured with ConA or SEB for 3 days in vitro.The subpopulation of splenic lymphocytes and its proliferation were detected using the flow cytometry and liquid scintillation analyzer,respectively.Results:After co-cultured with ConA or SEB in vitro,the percentage of CD4⁺T cells of splenic lymphocytes in SEB group significantly increased compared with the PBS group(P<0.05 to P<0.01).The percentages of CD4⁺TCR Vβ8.2⁺ T cells on the first day of culture,and CD8⁺TCR Vβ8.2⁺ T cells on the three day of culture in SEB group significantly increased compared with the contrd group(P<0.05 to P<0.01).Compared with PBS group,the stimulation of ConA or SEB significantly increased the proliferation ability of splenic lymphocytes in SEB group on the first day of culture,and significantly decreased on the second and third day of culture(P<0.05 to P<0.01).Conclusions:Maternal SEB administration during pregnancy can affect the proliferation of splenic lymphocytes in the newborn offspring rats in vitro.

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