Abstract: Objective:To investigate the effect of GRP94 gene on hepatocellular carcinomas cells.Methods:The siRNA sequence and negative control sequence of GRP94 were transfected to cells using lipidosome.The mRNA and protein expression levels of GRP94 in HepG2 cells were detected using qRT-PCR and Western blotting,respectively.The proliferation and apoptosis of cells were detected using CCK-8 and flow cytometry,respectively.The migration and invasion abilities of HepG2 cells were tested using wound healing and Transwell assay,respectively.Results:The mRNA and protein expression levels of GRP94 in HepG2 cells after transfecting siRNA-GRP94 sequence and NC sequence significantly decreased.After 48 h of transfection,the proliferation ability of cells significantly decreased,and the apoptosis rate of cells significantly increased.The scratch test showed that the 24 h healing rate of cells was significantly inhibited,and the Transwell assay showed that the number of migration and invasion cells significantly decreased.Conclusions:GRP94 gene can inhibit the apoptosis,and promote the proliferation,migration and invasion of HepG2 cells,which can provide an experiment basis in investigating the effects of GRP94 gene on the occurrence and development of hepatocellular carcinoma.