Abstract: Objective:To investigate the regulation of transient receptor potential vanilloid type 4(TRPV4) on calcium homeostasis in thoracic aortic endothelial cells of hypertensive mice.Methods:Intracellular calcium was labeled at the cellular and tissue level using calcium ion fluorescent probe Flou-4/AM,and the changes of calcium ion concentration of cells in high-salt diet group and normal diet group stimulated by the TRPV4-specific agonist GSK1016790A were measured.Results:The intracellular calcium concentration increased in the thoracic aortic endothelial cells of normal diet group and high-salt diet group stimulated by GSK1016790A to specifically activate the TRPV4 channel at cellular and tissue level(P<0.05).The increasing degree of the fluorescence intensity of calcium ion in high-salt diet group under the stimulation of GSK1016790A was lower than that in normal diet group(P<0.05).Conclusions:TRPV4 is involved in the regulation of calcium homeostasis in mouse thoracic aortic endothelial cells.Activating TRPV4 can promote the extracellular calcium influx into the cell to maintain the intracellular calcium homeostasis.In the pathological models,the effect of TRPV4 regulating intracellular calcium homeostasis is impaired in the hypertensive state induced by high-salt diet.