快速大容量尾静脉注射法建立肝细胞癌小鼠模型

秦中强; 周万飞; 李红俊; 刘明珠; 谈燚

doi:10.13898/j.cnki.issn.1000-2200.2018.11.003

快速大容量尾静脉注射法建立肝细胞癌小鼠模型

Establishment of mouse model of hepatocellular carcinoma using rapid and vast tail vein injection

摘要

摘要: 目的：通过快速大容量尾静脉注射法建立肝细胞癌小鼠模型。方法：采用快速大容量尾静脉注射法，将插入NRAS基因的NRASV12转座子质粒、表达myr-Akt基因转座子质粒以及转座酶SB100的混合盐溶液通过小鼠尾静脉大容量（2 mL）、短时间（7 s）进行注射，作为观察组；同时设置对照组，同样方法注射等量0.9%氯化钠注射液。注射3~4周后，处死观察组及对照组小鼠，通过病理学检查小鼠成瘤情况。结果：观察组小鼠成瘤率100%（24/24），经病理学检测为肝细胞癌；对照组小鼠无成瘤。结论：快速大容量尾静脉注射方法构建肝细胞癌小鼠模型方法简单，诱癌时间短，成功率高，重复性好。

Abstract: Objective: To establish the mouse model of hepatocellular carcinoma using rapid and vast tail vein injection.Methods: The mixed solution(2 mL) of transposon plasmid NRASV12 inserted by NRAS gene,transposon plasmid expressing activated AKT(myr-Akt) and transposase SB100 was injected into the tail vein of mice within 7 s(observation group).Mouse injected with the same volume saline was set as the control group.After 3 to 4 weeks of injection,the mice of the two groups were sacrificed,and the tumor formation was examined by pathology.Results: The tumor formation rate in observation group was 100%(24/24),and the hepatocellular carcinoma was identified by pathology.No tumor formation in control group was found.Conclusions: Establishment of the mouse model of hepatocellular carcinoma using rapid and vast tail vein injection is simple,has short cancer-inducing time,high success rate and good repeatability.

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