纳米纤维固相萃取高效液相色谱法测定人血浆中川芎嗪含量

邱新峰

doi:10.13898/j.cnki.issn.1000-2200.2019.06.023

纳米纤维固相萃取高效液相色谱法测定人血浆中川芎嗪含量

邱新峰

Determination of ligustrazine in human plasma using nanofiber-packed solid-phase extraction-high performance liquid chromatography

QIU Xin-feng

摘要

摘要:
目的建立纳米纤维固相萃取高效液相色谱法检测人血浆中川芎嗪含量。
方法样品经1%氨水稀释3倍，用活化后的PCAX型复合阴离子交换纳米纤维固相萃取柱净化富集，用去离子水淋洗，以50 μL甲醇洗脱，取20 μL进样检测。以甲醇1%冰醋酸水溶液（45：55，V/V）为流动相在岛津ODS-2色谱柱（250 mm×4.6 mm，5 μm）上进行分离，流速1.0 mL/min，检测波长295 nm。
结果该方法的线性范围为0.01~2.00 μg/mL，检出限为0.003 μg/mL，回收率为94.88%~103.11%，相对标准偏差为2.6%~4.3%。
结论该方法操作简单，具有样品需要量少、灵敏度高等优点，可用于人血浆中川芎嗪的含量测定。

Abstract:
ObjectiveTo establish a nanofiber-packed solid-phase extraction-high performance liquid chromatography to determine the serum level of ligustrazine.
MethodsThe sample was diluted by 1% ammonia solution for 3 times, purified and enriched with activated PCAX composite anion exchange nanofiber solid-phase extraction column, eluted with deionized water, eluted with 50 μL methanol, and 20 μL sample was detected.The methanol-1% acetic acid solution(45:55, V/V) was used as mobile phase for separation on Shimazu ODS-2 column(250 mm×4.6 mm, 5 μm) with flow rate for 1.0 mL/min and detection wavelength for 295 nm.
ResultsThe linear range of this method was from 0.01 to 2.00 μg/mL, the detection limit was 0.003 μg/mL, the recovery rate was from 94.88% to 103.11%, and the relative standard deviations was from 2.6% to 4.3%.
ConclusionsThe method has the advantages of simple operation, low sample requirement and high sensitivity, and can be used to determine the content of ligustrazine in human plasma.

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