解为慈. 萆苓祛痛方对糖尿病痛风模型大鼠肾脏HMGB1及FOXO3的影响[J]. 蚌埠医学院学报, 2020, 45(2): 170-173. DOI: 10.13898/j.cnki.issn.1000-2200.2020.02.009
    引用本文: 解为慈. 萆苓祛痛方对糖尿病痛风模型大鼠肾脏HMGB1及FOXO3的影响[J]. 蚌埠医学院学报, 2020, 45(2): 170-173. DOI: 10.13898/j.cnki.issn.1000-2200.2020.02.009
    XIE Wei-ci. Effect of Biling Qutong prescription on the levels of HMGB1 and FOXO3 in kidney of diabetic gout model rats[J]. Journal of Bengbu Medical College, 2020, 45(2): 170-173. DOI: 10.13898/j.cnki.issn.1000-2200.2020.02.009
    Citation: XIE Wei-ci. Effect of Biling Qutong prescription on the levels of HMGB1 and FOXO3 in kidney of diabetic gout model rats[J]. Journal of Bengbu Medical College, 2020, 45(2): 170-173. DOI: 10.13898/j.cnki.issn.1000-2200.2020.02.009

    萆苓祛痛方对糖尿病痛风模型大鼠肾脏HMGB1及FOXO3的影响

    Effect of Biling Qutong prescription on the levels of HMGB1 and FOXO3 in kidney of diabetic gout model rats

    • 摘要:
      目的观察萆苓祛痛方(萆苓方)对糖尿病痛风模型大鼠肾脏高迁移率族蛋白B1(HMGB1)及FOXO3的影响。
      方法选取健康雄性大鼠,分为正常对照组、模型组、萆苓组、吲哚美辛组和吡格列酮组,各8只。除正常对照组外,其他各组予高脂饲料喂养,联合小剂量链脲佐菌素35 mg/kg腹腔注射1次,以血糖≥16.7 mmol/L为成功建立糖尿病模型。4 d后关节腔注射5%尿酸钠溶液1次,诱导痛风模型。建模成功后萆苓组、吲哚美辛组和吡格列酮组继续给予相应药物21 d,正常对照组和模型组给予等量0.9%氯化钠溶液。21 d后,各组大鼠取血测定血糖、血脂、血尿酸,并取大鼠肾脏,测定HMGB1及FOXO3表达量。
      结果与正常对照组比较,模型组大鼠血糖、血尿酸、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平均明显升高(P < 0.01),肾组织HMGB1、FOXO3表达量均明显升高(P < 0.01)。与模型组比较,萆苓方组、吡格列酮组和吲哚美辛组大鼠血糖、血尿酸水平均下降(P < 0.05~P < 0.01),肾组织HMGB1、FOXO3相对表达量均明显降低(P < 0.01),萆苓方组和吡格列酮组TG、TC、LDL-C亦均下降(P < 0.05~P < 0.01),吲哚美辛组TC、LDL-C均下降(P < 0.05和P < 0.01)。
      结论萆苓方具有良好的降糖、调脂、降低血尿酸的作用,其作用机制可能与干预糖尿病痛风大鼠肾脏HMGB1及FOXO3的高表达有关,其对糖尿病痛风引起的肾脏病防治有重要意义。

       

      Abstract:
      ObjectiveTo observe the effects of Biling Qutong prescription(Biling prescription) on the levels of high mobility group B1(HMGB1) and FOXO3 in kidney of diabetic gout model rats.
      MethodsForty healthy rats were divided into the normal control group, model group, Biling group, indomethacin group and pioglitazone group(8 rats in each group).Except the normal control group, the other groups were fed with high-fat diet combined with low-dose streptozotocin 35 mg/kg once a day, and the blood glucose ≥"16.7 mmol/L was set as the standard of the diabetes model.After 4 days, the 0.9% sodium chloride solution was injected into the joint cavity of rats to establish the gout model.After the model was successfully established, the Biling group, indomethacin and pioglitazone group were continuously treated with relative drug for 21 days.The normal control group and model group were given the same amount of 0.9% sodium chloride solution.After 21 days, the levels of blood glucose, uric acid and blood uric acid, and expression levels of HMGB1 and FOXO3 in kidney were detected.
      ResultsCompared with the normal control group, the levels of blood glucose, serum uric acid, triacylglycerol(TG), total cholesterol (TC), low-density lipoprotein cholesterol(LDL-C) significantly increased(P < 0.01), and the expression levels of HMGB1 and FOXO3 in kidney significantly increased in model group(P < 0.01).Compared with the model group, the levels of blood glucose and serum uric acid decreased, and the expression levels of HMGB1 and FOXO3 in kidney significantly decreased in Biling group, indomethacin and pioglitazone group(P < 0.05 to P < 0.01).Compared with the model group, the levels of TG, TC and LDL-C in Biling group and pioglitazone group decreased(P < 0.05 to P < 0.01), and the levels of TC and LDL-C in indomethacin group decreased(P < 0.05 and P < 0.01).
      ConclusionsBiling prescription has good effects in lowering blood glucose, regulating lipid and lowering blood uric acid, the action mechanism of which may be related to the intervention of the high expression of HMGB1 and FOXO3 in kidney of diabetic gout rats, and which is of great significance for the prevention and treatment of kidney disease induced by diabeticgout.

       

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