马方昊, 靳丽杰, 叶国柳, 杜丹丽. miR-655在上皮性卵巢癌中的表达研究[J]. 蚌埠医学院学报, 2020, 45(2): 181-184. DOI: 10.13898/j.cnki.issn.1000-2200.2020.02.012
    引用本文: 马方昊, 靳丽杰, 叶国柳, 杜丹丽. miR-655在上皮性卵巢癌中的表达研究[J]. 蚌埠医学院学报, 2020, 45(2): 181-184. DOI: 10.13898/j.cnki.issn.1000-2200.2020.02.012
    MA Fang-hao, JIN Li-jie, YE Guo-liu, DU Dan-li. Study on the miR-655 expression in epithelial ovarian cancer[J]. Journal of Bengbu Medical College, 2020, 45(2): 181-184. DOI: 10.13898/j.cnki.issn.1000-2200.2020.02.012
    Citation: MA Fang-hao, JIN Li-jie, YE Guo-liu, DU Dan-li. Study on the miR-655 expression in epithelial ovarian cancer[J]. Journal of Bengbu Medical College, 2020, 45(2): 181-184. DOI: 10.13898/j.cnki.issn.1000-2200.2020.02.012

    miR-655在上皮性卵巢癌中的表达研究

    Study on the miR-655 expression in epithelial ovarian cancer

    • 摘要:
      目的探讨微小RNA(microRNA,miR)-655在上皮性卵巢癌中的表达、作用及其可能的分子机制。
      方法采用实时定量-PCR检测miR-655在40对上皮性卵巢癌组织和正常组织中的表达,分析miR-655表达水平与临床病理之间的关系。Cell Counting Kit-8(CCK-8)和流式细胞术检测miR-655对卵巢癌细胞增殖及凋亡的影响。Western blotting检测PI3K、AKT和mTOR蛋白的表达。
      结果miR-655在上皮性卵巢癌组织中的表达量明显低于正常组织(P < 0.01)。miR-655表达水平在不同肿瘤直径、病理分化程度和是否淋巴结转移病人间差异均有统计学意义(P < 0.05~P < 0.01),而不同年龄、CA-125病人间miR-655 mimics表达差异均无统计学意义(P>0.05)。转染miR-655 mimics可明显抑制卵巢癌细胞的增殖并促进细胞的凋亡(P < 0.01);miR-655 mimics转染明显抑制卵巢癌细胞中PI3K、AKT和mTOR活性(P < 0.01)。
      结论miR-655在卵巢癌组织中明显低表达,miR-655低表达可能与卵巢癌的肿瘤生长和分化程度相关,miR-655可明显抑制卵巢癌细胞的增殖,并促进其凋亡,其在卵巢癌病人中具有一定的靶向治疗价值。

       

      Abstract:
      ObjectiveTo investigate the expression level, function and possible molecular mechanism of microRNA (miR)-655 in epithelial ovarian cancer.
      MethodsThe expression levels of miR-655 in 40 cases of epithelial varian cancer tissues and normal tissues were detected using real-time quantitative-PCR, and the relationship between miR-655 expression and clinicopathological features was analyzed.The cell counting Kit-8(CCK-8) and flow cytometry were used to detect the effects of miR-655 on the proliferation and apoptosis of ovarian cancer cells.Western blotting was used to detect the expression of PI3K, AKT and mTOR.
      ResultsThe expression level of miR-655 in epithelial ovarian carcinoma was significantly lower than that in normal tissues(P < 0.01).The differences of the expression levels of miR-655 among patients with different tumor diameter, degree of pathological differentiation and lymph node metastasis were statistically significant(P < 0.05 to P < 0.01), and the differences of the expression levels of miR-655 among patients with different ages and CA-125 were not statistically significant(P>0.05).The transfection of miR-655 mimics could significantly inhibit the proliferation and promote apoptosis of ovarian cancer cells(P < 0.01).The transfection of MiR-655 mimics significantly could significantly inhibit the expression of PI3K, AKT and mTOR in ovarian cancer cells(P < 0.01).
      ConclusionsThe low expression of miR-65 in ovarian cancer tissue is significant, which may be related to the growth and differentiation of ovarian cancer.miR-655 can significantly inhibit the proliferation and induce apoptosis of ovarian cancer cells.miR-655 has a certain value of target therapy in ovarian cancer patients.

       

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