PD-L1对肝细胞癌细胞增殖、侵袭及迁移的影响

周万飞; 刘高峰; 秦中强; 胡小梅; 谈燚

doi:10.13898/j.cnki.issn.1000-2200.2020.05.002

PD-L1对肝细胞癌细胞增殖、侵袭及迁移的影响

Effect of PD-L1 on the proliferation,invasion and migration of hepatocellular carcinoma cells

摘要

摘要:
目的探讨程序性死亡配体(PD-L1)在肝癌细胞增殖、侵袭、迁移中的作用。

方法转染有效的PD-L1基因siRNA干扰片段进入人肝细胞癌HepG2细胞中，下调细胞中PD-L1表达。采用荧光定量PCR法检测HepG2细胞中PD-L1基因表达情况，采用Western blotting法检测HepG2细胞中PD-L1蛋白表达情况。通过MTT实验观察其对HepG2细胞增殖能力的影响，采用细胞划痕实验检测细胞融合率，采用Transwell小室进行侵袭实验，观察其对HepG2细胞迁移及侵袭能力影响。

结果下调PD-L1后，HepG2细胞PD-L1基因表达量、PD-L1蛋白表达量和细胞增殖能力、迁移能力及侵袭能力均明显低于阴性对照组和空白对照组(P＜0.01)，阴性对照组和正常对照组上述指标差异均无统计学意义(P＞0.05)。

结论下调PD-L1后，肝癌细胞的增殖、迁移、侵袭能力有一定程度下降，PD-L1可能成为肝细胞肝癌免疫治疗中的一个新基因靶位。

Abstract:
ObjectiveTo investigate the effects of PD-L1 in the proiferation, invasion and migration of hepatacellular carcinorma cells.
MethodsThe effective PD-L1 gene siRNA interference fragment was transfected into the human hepatocellular carcinoma HepG2 cells to down-regulate PD-L1 expression.The expression levels of PD-L1 gene and protein in HepG2 cells were detected using the real-time PCR and Western blotting, resepctively.The cells proliferation and fusion rate of HepG2 cells were observed using the MTT assay and cell scratch test, respectively.The invasion experiment was implemented using Transwell chamber, and the migration and invasion ability of HepG2 cells were observed.
ResultsAfter the expression level of PD-L1 gene was down-regulated, the expression levels of PD-L1 gene and protein in HepG2 cells, and proliferation, migration and invasion abilities of HepG2 cells in PD-L1 interference group were significantly lower than those in negative control group and blank control group(P＜0.01), and there was no statistical significance in the above indicators between the negative control group and normal control group(P＞0.05).
ConclusionsAfter the down-regulation of PD-L1, the proliferation, migration and invasion abilities of the human hepatocellular carcinoma cells decrease to a certain extent, and PD-L1 may become a new gene target in the immunotherapy of hepatocellular carcinoma.

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