精索静脉曲张的不育病人精子线粒体DNA片段缺失的调查

呼明闯; 章宏祥; 周发友; 高攀; 唐晓磊

doi:10.13898/j.cnki.issn.1000-2200.2020.06.029

精索静脉曲张的不育病人精子线粒体DNA片段缺失的调查

Investigation of the DNA fraction deficiency of sperm mitochondria in infertility patients with varicocele of spermatic cord

摘要

摘要:
目的分析精索静脉曲张导致的不育病人的精子线粒体DNA（mitochondrial DNA，mtDNA）中一个长为4 977 bp的片段缺失的情况。
方法收集60例精索静脉曲张的不育病人和90名健康男性的精液标本，并抽提精子的总DNA，用Gap-PCR技术检测DNA 4 977 bp片段的缺失情况。
结果在81.66%的患有精索静脉曲张的不育病人和4.44%的健康人对照中存在DNA 4 977 bp片段的缺失。患有精索静脉曲张的不育病人中DNA缺失频率更高（OR=139.80，95%CI=40.08~487.30，P < 0.01）。
结论精索静脉曲张可能会导致一些男性精子DNA缺失，并导致不育。且精子线粒体DNA 4 977 bp片段缺失与精索静脉曲张所致不育症存在一定的相关性。

Abstract:
ObjectiveTo analyze the 4977bp sperm mitochondrial DNA(mtDNA) deficiency in patient with varicocele infertility.
MethodsThe semen samples of 60 infertile patients with clinical varicocele and 90 normal men were collected.The total DNA of all cases were extracted, and the 4977bp sperm mtDNA deficiency was detected using the Gap-PCR.
ResultsThe 4977 bp DNA deficiency in 81.66% of patients with varicocele infertility and 4.44% of healthy people was identified.The DNA missing frequency in infertile patients with varicocele was more(OR=139.80, 95%CI=40.08-487.30).
ConclusionsThe varicocele may induce the mtDNA deficiency of sperm and infertility.The 4977 bp mtDNA deficiency of sperm is correlated with the infertility caused byvaricocele.

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