程静静, 余丽丽, 周勇兵, 汤雨婷, 马雨妍, 吴俊英, 刘辉. 芦丁减轻全氟辛酸暴露致小鼠心肌损伤的作用研究[J]. 蚌埠医学院学报, 2020, 45(11): 1462-1466. DOI: 10.13898/j.cnki.issn.1000-2200.2020.11.003
    引用本文: 程静静, 余丽丽, 周勇兵, 汤雨婷, 马雨妍, 吴俊英, 刘辉. 芦丁减轻全氟辛酸暴露致小鼠心肌损伤的作用研究[J]. 蚌埠医学院学报, 2020, 45(11): 1462-1466. DOI: 10.13898/j.cnki.issn.1000-2200.2020.11.003
    CHENG Jing-jing, YU Li-li, ZHOU Yong-bing, TANG Yu-ting, MA Yu-yan, WU Jun-ying, LIU Hui. Effect of rutin on reducing myocardial injury induced by perfluorooctanoic acid exposure in mice[J]. Journal of Bengbu Medical College, 2020, 45(11): 1462-1466. DOI: 10.13898/j.cnki.issn.1000-2200.2020.11.003
    Citation: CHENG Jing-jing, YU Li-li, ZHOU Yong-bing, TANG Yu-ting, MA Yu-yan, WU Jun-ying, LIU Hui. Effect of rutin on reducing myocardial injury induced by perfluorooctanoic acid exposure in mice[J]. Journal of Bengbu Medical College, 2020, 45(11): 1462-1466. DOI: 10.13898/j.cnki.issn.1000-2200.2020.11.003

    芦丁减轻全氟辛酸暴露致小鼠心肌损伤的作用研究

    Effect of rutin on reducing myocardial injury induced by perfluorooctanoic acid exposure in mice

    • 摘要:
      目的探讨芦丁对全氟辛酸(PFOA)造成的小鼠心肌损伤的保护作用研究。
      方法采用PFOA暴露建立小鼠心肌损伤模型,观察芦丁对PFOA造成小鼠心肌损伤模型体质量、心脏质量、心肌TG、TC含量和血清LDH活性、氧化应激指标变化(心肌MDA含量、GSH-PX和T-SOD活性)及心肌组织形态学变化。
      结果各组小鼠体质量1~2 d比较差异无统计学意义(P>0.05);3~14 d各组小鼠体质量有差异,且PFOA组小鼠体质量低于PFOA+芦丁组(P < 0.05);PFOA组和PFOA+芦丁组小鼠干预14 d心脏质量低于对照组,而PFOA+芦丁组高于PFOA组(P < 0.05)。PFOA组小鼠14 d心肌TC含量及血清LDH活性高于对照组(P < 0.05);PFOA+芦丁组小鼠14 d心肌TG含量及血清LDH活性低于PFOA组(P < 0.05);而PFOA组与PFOA+芦丁组小鼠14 d心肌TC含量差异无统计学意义(P>0.05);PFOA组小鼠14 d心肌MDA含量高于对照组(P < 0.05),而PFOA组与对照组小鼠14 d心肌T-SOD、GSH-PX活性差异无统计学意义(P>0.05);PFOA+芦丁组小鼠14 d心肌GSH-PX活性高于PFOA组,而PFOA+芦丁组与PFOA组在小鼠14 d时心肌MDA含量、GSH-PX活性差异无统计学意义(P>0.05)。
      结论芦丁干预可以减轻PFOA暴露致小鼠心肌损伤作用。

       

      Abstract:
      ObjectiveTo explore the protective effects of rutin on myocardial injury induced by perfluorooctanoic acid(PFOA) exposure in mice.
      MethodsPFOA was used to establish a model of myocardial injury in mice, and the effects of rutin on the body weight, heart weight, myocardial TG, TC content, serum LDH activity, and oxidative stress indicators(myocardial MDA content, GSH-PX and T-SOD activity) and myocardial morphology were investigated in mice.
      ResultsThere was no statistical significance in the weight of 1-2 d mice among all groups(P>0.05).The differences of in the weight of 3- 14 d mice on day 8 to day 14 among all groups were statistically significant(P < 0.05), and the weight of mice in PFOA group was lower than that in PFOA+rutin group, the heart weight of mice in PFOA group and PFOA+rutin group was lower than that in control group after 14 days of intervention, while the heart weight of mice in PFOA+rutin group was higher than that in PFOA group(P < 0.05).After 14 days of intervention, the myocardial TC content and serum LDH activity in PFOA group were higher than those in control group(P < 0.05), the myocardial TG content and serum LDH activity in PFOA+rutin group were lower than those in PFOA group(P < 0.05), and the difference of the myocardial TC content between PFOA group and PFOA+rutin group was not statistically significant(P>0.05).After 14 days of inverention, the myocardial MDA content and in PFOA group was higher than those in control group(P < 0.05), but there was no statistical significance in myocardial GSH-PX activity between PFOA group and the control group(P>0.05), the myocardial MDA content in PFOA+rutin group was lower than that in PFOA group, while the differences of the myocardial MDA content and GSH-PX activity between PFOA+rutin group and PFOA group were not statistically significant(P>0.05).
      ConclusionsRutin intervention can ameliorate the myocardial injury induced by PFOA exposure in mice.

       

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