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    周轶, 王英媛. 山茶油对哮喘患儿外周血单个核细胞GATA-3表达和IL-4分泌的影响[J]. 蚌埠医科大学学报, 2020, 45(12): 1602-1605, 1610. DOI: 10.13898/j.cnki.issn.1000-2200.2020.12.003
    引用本文: 周轶, 王英媛. 山茶油对哮喘患儿外周血单个核细胞GATA-3表达和IL-4分泌的影响[J]. 蚌埠医科大学学报, 2020, 45(12): 1602-1605, 1610. DOI: 10.13898/j.cnki.issn.1000-2200.2020.12.003
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    ZHOU Yi, WANG Ying-yuan. Effect of camellia oil on GATA-3 expression and IL-4 secretion in peripheral blood mononuclear cells of children with asthma[J]. Journal of Bengbu Medical University, 2020, 45(12): 1602-1605, 1610. DOI: 10.13898/j.cnki.issn.1000-2200.2020.12.003
    Citation: ZHOU Yi, WANG Ying-yuan. Effect of camellia oil on GATA-3 expression and IL-4 secretion in peripheral blood mononuclear cells of children with asthma[J]. Journal of Bengbu Medical University, 2020, 45(12): 1602-1605, 1610. DOI: 10.13898/j.cnki.issn.1000-2200.2020.12.003
    shu

    山茶油对哮喘患儿外周血单个核细胞GATA-3表达和IL-4分泌的影响

    Effect of camellia oil on GATA-3 expression and IL-4 secretion in peripheral blood mononuclear cells of children with asthma

      摘要
    • 摘要:
      目的探讨山茶油对哮喘患儿外周血单个核细胞（PBMCs）转录因子GATA结合蛋白3（GATA-3）表达和白细胞介素-4（IL-4）分泌的影响。
      方法采用密度梯度离心法分离哮喘患儿肝素化血液中的PBMCs，以10、50、100、200 μmol/L的山茶油处理PBMCs 24 h。MTT法检测山茶油对PBMCs活性的影响；ELISA检测山茶油对PBMCs IL-4分泌的影响；实时荧光定量PCR检测山茶油处理PBMCs后GATA-3 mRNA和IL-4 mRNA的表达水平；Western blotting检测山茶油处理PBMCs中GATA-3蛋白表达水平。
      结果与对照组比较，10、50、100 μmol/L山茶油对PBMCs活力无明显影响（P>0.05），200 μmol/L山茶油能明显降低PBMCs活力（P < 0.01）；不同浓度山茶油处理PBMCs后，IL-4分泌水平均明显降低（P < 0.01），当山茶油浓度为50 μmol/L时，IL-4分泌水平最低（P < 0.01）；不同浓度山茶油处理PBMCs后，GATA-3 mRNA和IL-4 mRNA的表达均被明显抑制（P < 0.05），当山茶油浓度为50 μmol/L时，GATA-3与IL-4的mRNA表达量最低（P < 0.05）；不同浓度山油茶处理PBMCs后GATA-3蛋白表达均降低（P < 0.05），当山茶油浓度为10 μmol/L时，GATA-3蛋白的表达量达到最低水平（P < 0.05）。
      结论山茶油可抑制哮喘患儿PBMCs中GATA-3表达及IL-4的分泌，对哮喘具有潜在的治疗作用。

       

      Abstract:
      ObjectiveTo investigate the effect of camellia oil on GATA-binding protein 3(GATA-3)expression and interleukin 4(IL-4)secretion in peripheral blood mononuclear cells(PBMCs)of children with asthma.
      MethodsPBMCs in heparinized blood of asthmatic children were separated by density gradient centrifugation.PBMCs were treated with 10, 50, 100 and 200 μmol/L camellia oil for 24 h.MTT assay was used to detect the effect of camellia oil on the activity of PBMCs, ELISA was applied to determine the effect of camellia oil on the IL-4 secretion, real-time fluorescence quantitative PCR was employed to measure the expression of GATA-3 mRNA and IL-4 mRNA, and Western blotting was performed to analyze the expression of GATA-3 protein in PBMCs treated with camellia oil.
      ResultsCompared with control group, 10, 50 and 100 μmol/L camellia oil had no significant effect on the activity of PBMCs(P>0.05), but 200 μmol/L camellia oil could significantly reduce the activity of PBMCs(P < 0.01);the secretion level of IL-4 in PBMCs treated with different concentrations of camellia oil decreased significantly(P < 0.01), and 50 μmol/L camellia oil had the best effect(P < 0.01);the expression of GATA-3 mRNA and IL-4 mRNA in PBMCs treated with different concentrations of camellia oil were significantly inhibited(P < 0.05), and 50 μmol/L camellia oil had the best effect(P < 0.05);the expression of GATA-3 protein in PBMCs treated with different concentrations of camellia oil was decreased(P < 0.05), and 10 μmol/L camellia oil had the best effect(P < 0.05).
      ConclusionsCamellia oil can inhibit GATA-3 expression and IL-4 secretion in PBMCs of asthmatic children, and has potential therapeutic effect on asthma.

       

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