IL-33及其受体ST2对人卵巢癌ES-2细胞增殖、凋亡、迁移和侵袭的影响

缪晨婷; 钱静; 朱璟

doi:10.13898/j.cnki.issn.1000-2200.2020.12.004

IL-33及其受体ST2对人卵巢癌ES-2细胞增殖、凋亡、迁移和侵袭的影响

Effect of IL-33 and its receptor ST2 on the proliferation, apoptosis, migration and invasion of human ovarian cancer ES-2 cells

摘要

摘要:
目的探讨白细胞介素-33（IL-33）及其受体磺基转移酶（ST2）对人卵巢癌ES-2细胞增殖、凋亡、迁移和侵袭的影响。
方法采用MTT实验检测IL-33和si-ST2对ES-2人卵巢癌细胞存活率的影响；分为对照组、IL-33组、si-ST2组以及IL-33+si-ST2组，检测各组吸光度值；用流式细胞术检测IL-33和si-ST2对ES-2人卵巢癌细胞凋亡的影响，统计各组细胞凋亡数目；用细胞迁移和侵袭实验检测IL-33和si-ST2对ES-2人卵巢癌细胞迁移、侵袭的影响。
结果IL-33组、si-ST2组、IL-33+si-ST2组以及对照组的细胞存活率的比较差异有统计学意义（P < 0.01）。其中IL-33组细胞存活率高于对照组，si-ST2组细胞存活率低于对照组，IL-33+si-ST2组细胞存活率显著高于si-ST2组，IL-33组细胞存活率显著高于si-ST2组，差异均有统计学意义（P < 0.01）。IL-33组细胞凋亡率低于对照组；si-ST2组细胞凋亡率高于对照组和IL-33组；IL-33+si-ST2组细胞凋亡率低于si-ST2组，高于IL-33组。IL-33组、si-ST2组、IL-33+si-ST2组以及对照组的细胞迁移率、侵袭率的比较，差异有统计学意义（P < 0.01）。其中IL-33组细胞迁移、侵袭均低于对照组，si-ST2组迁移、侵袭均高于空白对照组，IL-33+si-ST2组迁移、侵袭均高于IL-33组，低于si-ST2组，差异均有统计学意义（P < 0.01）。
结论IL-33可促进卵巢癌ES-2细胞增殖并抑制其凋亡、迁移、侵袭；沉默ST2可抑制卵巢癌ES-2细胞增殖并促进其凋亡，使其迁移和侵袭能力下降；IL-33通过ST2途径参与影响卵巢癌ES-2细胞的增殖、凋亡、迁移和侵袭。

Abstract:
ObjectiveTo investigate the effects of interleukin-33(IL-33) and its receptor sulfotransferase(ST2) on the proliferation, apoptosis, migration and invasion of human ovarian cancer ES-2 cells.
MethodsThe effects of IL-33 and si-ST2 on the survival rate of ES-2 cells were detected using MTT assay, and the cells were divided into the control group, IL-33 group, si-ST2 group and IL-33+si-ST2 group.The OD value in each group was detected.The effects of IL-33 and si-ST2 on the apoptosis of ES-2 cells were detected using flow cytometry, and the number of apoptotic cells in each group was counted.The effects of IL-33 and si-ST2 on the migration and invasion of ES-2 cells were detected using the cell migration and invasion experiments.
ResultsThe differences of cell survival rates among the IL-33 group, si-ST2 group, IL-33+si-ST2 and control group were statistically significant(P < 0.01).The cell survival rate in IL-33 group was higher than that in control group, the cell survival rate in si-ST2 group was lower than that in control group, the cell survival rate in IL-33+si-ST2 group was significantly higher than that in si-ST2 group, and the cell survival rate in IL-33 group was significantly higher than that in si-ST2 group(P < 0.01).The cell apoptosis rate in IL-33 group was lower than that in control group, the cell apoptosis rate in si-ST2 group was higher than that in control group and IL-33 group, and the cell apoptosis rate in IL-33+si-ST2 group was lower than that in si-ST2 group, and higher than that in IL-33 group(P < 0.01).The differences of the cell migration rate and invasion rate among the IL-33 group, si-ST2 group, IL-33+si-ST2 group and control group were statistically significant(P < 0.01).The migration and invasion rates of cells in IL-33 group were lower than those in control group, the migration and invasion rates of cells in si-ST2 group were higher than those in control group, and the migration and invasion rates of cells in IL-33+si-ST2 group were higher than those in IL-33 group, and lower than those in si-ST2 group(P < 0.01).
ConclusionsIL-33 can promote the proliferation, and inhibit the apoptosis, migration and invasion of ES-2 cells.Silencing ST2 can inhibit the proliferation, and promote the apoptosis, migration and invasion of ES-2 cells.IL-33 affects the proliferation, apoptosis, migration and invasion of ES-2 cells through ST2 pathway.

HTML全文

参考文献(17)

施引文献

资源附件(0)