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    周中明, 严小虎, 梁磊. miR-432、miR-646和miR-100在骨肉瘤细胞中的表达[J]. 蚌埠医科大学学报, 2020, 45(12): 1646-1650, 1654. DOI: 10.13898/j.cnki.issn.1000-2200.2020.12.014
    引用本文: 周中明, 严小虎, 梁磊. miR-432、miR-646和miR-100在骨肉瘤细胞中的表达[J]. 蚌埠医科大学学报, 2020, 45(12): 1646-1650, 1654. DOI: 10.13898/j.cnki.issn.1000-2200.2020.12.014
    shu
    ZHOU Zhong-ming, YAN Xiao-hu, LIANG Lei. Expression and clinical significance of miR-432, miR-646 and miR-100 in osteosarcoma cells[J]. Journal of Bengbu Medical University, 2020, 45(12): 1646-1650, 1654. DOI: 10.13898/j.cnki.issn.1000-2200.2020.12.014
    Citation: ZHOU Zhong-ming, YAN Xiao-hu, LIANG Lei. Expression and clinical significance of miR-432, miR-646 and miR-100 in osteosarcoma cells[J]. Journal of Bengbu Medical University, 2020, 45(12): 1646-1650, 1654. DOI: 10.13898/j.cnki.issn.1000-2200.2020.12.014
    shu

    miR-432、miR-646和miR-100在骨肉瘤细胞中的表达

    Expression and clinical significance of miR-432, miR-646 and miR-100 in osteosarcoma cells

      摘要
    • 摘要:
      目的探讨miR-432、miR-646和miR-100在骨肉瘤细胞中的表达及临床意义。
      方法选择行根治性切除手术的72例骨肉瘤组织作为研究对象，同时选择38例骨肉瘤旁组织作为对照组。采用RT-qPCR的方法检测各组组织细胞中miR-432、miR-646和miR-100的表达水平与骨肉瘤病人临床病理参数的关系，并分析miR-432、miR-646和miR-100的表达水平之间的相关性。
      结果骨肉瘤组的miR-432、miR-646和miR-100的表达水平低于骨肉瘤旁组织（P < 0.01）。骨肉瘤组织细胞中miR-432、miR-646和miR-100的表达水平与病人的性别、年龄、肿瘤直径、组织学类型、发病部位和TCNR均无关（P>0.05），而与病人的TNM分期和是否发生肺转移相关，miR-432和miR-100表达量随着TNM分期增加而降低，miR-646表达量Ⅲ期低于Ⅰ期和Ⅱ期，发生肺转移病人miR-432、miR-646和miR-100表达量均低于非肺转移病人（P < 0.05~P < 0.01）。Pearson相关性分析，miR-432的表达水平与miR-646和miR-100的表达水平正相关（r=0.76和0.79，P < 0.05），而肿瘤病人中miR-646的表达水平与miR-100的表达水平呈正相关（r=0.81，P < 0.01）。
      结论miR-432、miR-646和miR-100在骨肉瘤组织细胞中异常表达，并且与骨肉瘤的发生及发展密切相关，为临床上骨肉瘤的诊断和治疗提供依据。

       

      Abstract:
      ObjectiveTo investigate the expression levels and clinical significance of miR-432, miR-646 and miR-100 in osteosarcoma cells.
      MethodsSeventy-two cases of osteosarcoma treated with radical resection and 38 cases of adjacent tissue to osteosarcoma were divided into the osteosarcoma group and control group, respectively.The expression levels of miR-432, miR-646 and miR-100 in two groups were detected using RT-qPCR, and the correlation between the expression levels of miR-432, miR-646 and miR-100, and clinicopathological parameters of patients with osteosarcoma were analyzed.
      ResultsThe expression levels of miR-432, miR-646 and miR-100 in osteosarcoma group were lower than those in osteosarcoma tissues(P < 0.01).The expression levels of miR-432, miR-646, and miR-100 in osteosarcoma tissue cells were not related to the patient's gender, age, tumor diameter, histological type, disease site and TCNR, but the patient's TNM stage was related to the occurrence of lung metastases.The expression levels of miR-432 and miR-100 decreased with the increasing of TNM stage.The expression level of miR-646 in stage Ⅲ was lower than that in stagesⅠand Ⅱ.The expression levels of miR-432, miR-646 and miR-100 in patients with pulmonary metastases were lower than those in patients without pulmonary metastases(P < 0.05 to P < 0.01).The results of Pearson correlation analysis showed that the expression level of miR-432 was positively correlated with the expression levels of miR-646 and miR-100(r=0.76 and 0.79, P < 0.05), while the expression level of miR-646 in tumor patients was positively related to the expression level of miR-100(r=0.81, P < 0.01).
      ConclusionsThe expressions of miR-432, miR-646 and miR-100 are abnormal in osteosarcoma tissue cells, and closely related to the occurrence and development of osteosarcoma, which can provide a basis for the diagnosis and treatment of osteosarcoma in clinic.

       

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