高级检索
    张云霄, 任婷, 叶苗苗, 王元元, 陶静, 李言. 川芎嗪对脂多糖诱导的人脐静脉内皮细胞高通透性的保护作用研究[J]. 蚌埠医科大学学报, 2021, 46(2): 141-145. DOI: 10.13898/j.cnki.issn.1000-2200.2021.02.001
    引用本文: 张云霄, 任婷, 叶苗苗, 王元元, 陶静, 李言. 川芎嗪对脂多糖诱导的人脐静脉内皮细胞高通透性的保护作用研究[J]. 蚌埠医科大学学报, 2021, 46(2): 141-145. DOI: 10.13898/j.cnki.issn.1000-2200.2021.02.001
    shu
    ZHANG Yun-xiao, REN Ting, YE Miao-miao, WANG Yuan-yuan, TAO Jing, LI Yan. Protective effect of tetramethylpyrazine on lipopolysaccharide-induced high permeability of human umbilical vein endothelial cells[J]. Journal of Bengbu Medical University, 2021, 46(2): 141-145. DOI: 10.13898/j.cnki.issn.1000-2200.2021.02.001
    Citation: ZHANG Yun-xiao, REN Ting, YE Miao-miao, WANG Yuan-yuan, TAO Jing, LI Yan. Protective effect of tetramethylpyrazine on lipopolysaccharide-induced high permeability of human umbilical vein endothelial cells[J]. Journal of Bengbu Medical University, 2021, 46(2): 141-145. DOI: 10.13898/j.cnki.issn.1000-2200.2021.02.001
    shu

    川芎嗪对脂多糖诱导的人脐静脉内皮细胞高通透性的保护作用研究

    Protective effect of tetramethylpyrazine on lipopolysaccharide-induced high permeability of human umbilical vein endothelial cells

      摘要
    • 摘要:
      目的观察川芎嗪（tetramethylpyrazine, TMP）对脂多糖（lipopolysaccharide, LPS）诱导的人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVECs）通透性变化和细胞骨架相关蛋白表达影响。
      方法以体外培养的HUVECs为实验对象，将细胞随机分6组：正常对照组, LPS组（1μg/mL LPS）, TMP低、中、高剂量防治组（60μg/mL TMP+1μg/mL LPS、120μg/mL TMP+1μg/mL LPS、240μg/mL TMP+1μg/mL LPS）, 纯TMP组（240μg/mL TMP）。Transwell小室内建立内皮细胞通透性模型；细胞电压电阻仪测定各组细胞跨内皮细胞电阻值（TEER）；FITC-葡聚糖法检测各组细胞通透性变化，Pull-Down实验拉下Rac1-GTPase活性蛋白，Western blotting检测各组细胞Rac1-GTPase、Rac1、LIMK1和p-LIMK1的蛋白表达。
      结果3组不同浓度的TMP均可以抑制LPS诱导的内皮细胞TEER降低以及降低LPS导致的内皮细胞通透性的增加（P < 0.05~P < 0.01）；Western blotting实验显示TMP可以有效抑制LPS诱导的HUVECs中的Rac1-GTPase、p-LIMK1、Rac1、LIMK1表达（P < 0.05~P < 0.01）。
      结论TMP可以有效抑制LPS诱导的HUVECs通透性升高，其机制可能与其下调Rac1/LIMK1信号通路中Rac1-GTPase活性蛋白、LIMK1磷酸化蛋白表达有关。

       

      Abstract:
      ObjectiveTo investigate the effect of tetramethylpyrazine (TMP) on the permeability of human umbilical vein endothelial cells (HUVECs) induced by lipopolysaccharide (LPS) and the expression of cytoskeleton-related proteins.
      MethodsHUVECs cultured in vitro were randomly divide into normal control group, LPS group (1 μg/mL LPS), TMP low, medium and high dose group (60 μg/mL TMP+1 μg/mL LPS, 120 μg/mL TMP+1 μg/mL LPS, 240 μg/mL TMP+1 μg/mL LPS), pure TMP group (240 μg/mL TMP).Endothelial cell permeability model was established in Transwell chamber; cell voltage resistance meter was used to measure the trans-endothelial electrical resistance in each group; FITC-dextran method was used to detect the permeability change in each group, and Pull-Down experiment was used to collect Rac1-GTPase active protein, Western blotting was used to detect the protein expression of Rac1-GTPase, Rac1, LIMK1 and p-LIMK1 in each group.
      ResultsTEER and permeability coefficient showed that three different concentrations of TMP inhibited the decrease of TEER induced by LPS in endothelial cells and decreased LPS-induced endothelial cell permeability(P < 0.05 to P < 0.01);Western blotting analysis showed that TMP could effectively inhibit the LPS-induced up-regulation of Rac1-GTPase, p-LIMK1, Rac1 and LIMK1 in HUVECs(P < 0.05 to P < 0.01).
      ConclusionsTMP can effectively inhibit the increase of permeability of HUVECs induced by LPS, which may be related to the down-regulation of Rac1-GTPase active protein and LIMK1 phosphorylation protein expression in Rac1/LIMK1 signaling pathway.

       

      • HTML全文
      • 参考文献(14)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回