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    于宇, 李晓宁. LncRNA PCBP1-AS1对口腔鳞癌细胞增殖、侵袭和凋亡的影响[J]. 蚌埠医科大学学报, 2021, 46(2): 154-158. DOI: 10.13898/j.cnki.issn.1000-2200.2021.02.004
    引用本文: 于宇, 李晓宁. LncRNA PCBP1-AS1对口腔鳞癌细胞增殖、侵袭和凋亡的影响[J]. 蚌埠医科大学学报, 2021, 46(2): 154-158. DOI: 10.13898/j.cnki.issn.1000-2200.2021.02.004
    shu
    YU Yu, LI Xiao-ning. Effect of LncRNA PCBP1-AS1 on the proliferation, invasion and apoptosis of oral squamous carcinoma cells[J]. Journal of Bengbu Medical University, 2021, 46(2): 154-158. DOI: 10.13898/j.cnki.issn.1000-2200.2021.02.004
    Citation: YU Yu, LI Xiao-ning. Effect of LncRNA PCBP1-AS1 on the proliferation, invasion and apoptosis of oral squamous carcinoma cells[J]. Journal of Bengbu Medical University, 2021, 46(2): 154-158. DOI: 10.13898/j.cnki.issn.1000-2200.2021.02.004
    shu

    LncRNA PCBP1-AS1对口腔鳞癌细胞增殖、侵袭和凋亡的影响

    Effect of LncRNA PCBP1-AS1 on the proliferation, invasion and apoptosis of oral squamous carcinoma cells

      摘要
    • 摘要:
      目的探讨长链非编码RNA PCBP1-AS1（LncRNA PCBP1-AS1）对口腔鳞状细胞癌（OSCC）细胞增殖、侵袭及凋亡的影响及其可能机制。
      方法采用实时荧光定量聚合酶链反应检测OSCC细胞中PCBP1-AS1的表达水平；采用Lipofectamine 2000将pcDNA-PCBP1-AS1及pcDNA-control转染入口腔鳞癌CAL-27细胞；甲基噻唑基四唑实验检测CAL-27细胞的增殖能力；Transwell实验检测细胞的侵袭能力；流式细胞术检测细胞的凋亡率；蛋白免疫印迹法检测细胞周期依赖蛋白激酶1（CDK1）、基质金属蛋白酶-2（MMP-2）、B淋巴细胞瘤-2（Bcl-2）、B淋巴细胞瘤-2相关蛋白（Bax）、Janus激酶2（JAK2）/信号转导与转录激活因子3（STAT3）信号通路相关蛋白表达。
      结果与正常口腔上皮细胞比较，OSCC细胞CAL-27、Tca8113、KB中PCBP1-AS1的表达水平显著降低（P < 0.01）；PCBP1-AS1过表达可显著抑制CAL-27细胞的增殖、侵袭（P < 0.01），促进细胞凋亡（P < 0.01）；PCBP1-AS1过表达可显著抑制CDK1、MMP-2、Bcl2、p-JAK2、p-STAT3的蛋白表达（P < 0.01），而促进Bax的蛋白表达（P < 0.01）。
      结论PCBP1-AS1过表达可抑制OSCC细胞增殖、侵袭，诱导细胞凋亡，其作用可能与抑制JAK2/STAT3信号通路激活有关。

       

      Abstract:
      ObjectiveTo investigate the effects of long-chain non-coding RNA PCBP1-AS1(LncRNA PCBP1-AS1)on the proliferation, invasion and apoptosis of oral squamous carcinoma cells(OSCC)cells, and its possible mechanism.
      MethodsThe expression level of PCBP1-AS1 in OSCC cells was detected using Real-time quantitative polymerase chain reaction(qRT-PCR).The pcDNA-PCBP1-AS and pcDNA-control were transfected into the luminal squamous cell carcinoma CAL-27 cells using Lipofectamine 2000.The proliferation ability, invasive ability and apoptotic rate of CAL-27 cells were detected using MTT assay, Transwell experiments and flow cytometry, respectively.
      ResultsCompared with the normal oral epithelial cells, the expression levels of PCBP1-AS1 in oral squamous cell carcinoma cells CAL-27, Tca8113, and KB significantly decreased(P < 0.01).The overexpression of PCBP1-AS1 could significantly inhibit the proliferation and invasion of CAL-27 cells(P < 0.01), and promote apoptosis of cells(P < 0.01).The overexpression of PCBP1-AS1 could significantly inhibit the protein expression levels of CDK1, MMP-2, Bcl2, p-JAK2 and p-STAT3(P < 0.01), and promote the protein expression of Bax(P < 0.01).
      ConclusionsThe overexpression of PCBP1-AS1 can inhibit the proliferation and invasion of OSCC cells, and induce its apoptosis, and the mechanism of which may be related to the inhibition of JAK2/STAT3 signaling pathway activation.

       

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