Abstract:
ObjectiveTo explore the effects of siRNA silencing mucin 16(MUC16) on the proliferation and migration of gallbladder carcinoma GBC-SD cells, and its molecular mechanisms.
MethodsThe GBC-SD+MUC16-siRNA group was constructed using the lentivirus infection and siRNA interference technique to silence MUC16 gene in GBC-SD cells, and the negative control group(GBC-SD+NC group) and blank control group(GBC-SD group) were set up simultaneously.The RT-PCR was used to detect the expression of MUC16 in GBC-SD cells, the MTT and scratch assays were used to detect the proliferation and migration ability of GBC-SD cells, and the Western blotting was used to detect the protein expressions of CD44 and PCNA in GBC-SD cells.
ResultsThe cell proliferation rate and cell migration number in GBC-SD+MUC16-SiRNA group were lower than those in GBC-SD group(P < 0.05).The expression levels of MUC16 and CD44 protein in GBC-SD+MUC16-SIRNA group were lower than those in GBC-SD group and GBC-SD+NC group(P < 0.05 to P < 0.01), and the expression level of PCNA protein in GBC-SD+MUC16-SIRNA group was also lower than that in GBC-SD+NC group(P < 0.05).
ConclusionsSiRNA silencing MUC16 can inhibit the proliferation and migration of gallbladder cancer cells, which may provide the new experimental evidence for the treatment of gallbladder cancer.