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    夏泽亮, 刘言玉, 褚亮, 刘言浩, 王法宝, 周超毅, 蒋磊. siRNA沉默MUC16对胆囊癌细胞增殖和迁移的影响[J]. 蚌埠医科大学学报, 2021, 46(10): 1345-1348. DOI: 10.13898/j.cnki.issn.1000-2200.2021.10.005
    引用本文: 夏泽亮, 刘言玉, 褚亮, 刘言浩, 王法宝, 周超毅, 蒋磊. siRNA沉默MUC16对胆囊癌细胞增殖和迁移的影响[J]. 蚌埠医科大学学报, 2021, 46(10): 1345-1348. DOI: 10.13898/j.cnki.issn.1000-2200.2021.10.005
    shu
    XIA Ze-liang, LIU Yan-yu, CHU Liang, LIU Yan-hao, WANG Fa-bao, ZHOU Chao-yi, JIANG Lei. Effect of siRNA silencing MUC16 on the proliferation and migration of gallbladder carcinoma cells[J]. Journal of Bengbu Medical University, 2021, 46(10): 1345-1348. DOI: 10.13898/j.cnki.issn.1000-2200.2021.10.005
    Citation: XIA Ze-liang, LIU Yan-yu, CHU Liang, LIU Yan-hao, WANG Fa-bao, ZHOU Chao-yi, JIANG Lei. Effect of siRNA silencing MUC16 on the proliferation and migration of gallbladder carcinoma cells[J]. Journal of Bengbu Medical University, 2021, 46(10): 1345-1348. DOI: 10.13898/j.cnki.issn.1000-2200.2021.10.005
    shu

    siRNA沉默MUC16对胆囊癌细胞增殖和迁移的影响

    Effect of siRNA silencing MUC16 on the proliferation and migration of gallbladder carcinoma cells

      摘要
    • 摘要:
      目的探讨siRNA沉默黏蛋白(mucin，MUC)16基因对人胆囊癌细胞(GBC-SD)增殖和迁移的影响及其分子机制。
      方法采用慢病毒感染和siRNA干扰技术沉默GBC-SD中MUC16基因(GBC-SD+MUC16-siRNA组)，同时设立阴性对照组(GBC-SD+NC组)和空白对照组(GBC-SD组)。RT-PCR检测各组GBC-SD细胞中MUC16表达情况，噻唑蓝实验检测GBC-SD细胞增殖能力，划痕实验检测GBC-SD细胞迁移能力，蛋白免疫印迹实验检测GBC-SD细胞中PCNA和CD44蛋白表达情况。
      结果GBC-SD+MUC16-siRNA组细胞增殖率和细胞迁移数目均低于GBC-SD组(P < 0.05)；GBC-SD+MUC16-siRNA组MUC16、CD44蛋白表达均低于GBC-SD组、GBC-SD+NC组(P < 0.05~P < 0.01)，PCNA蛋白表达亦低于GBC-SD+NC组(P < 0.05)。
      结论siRNA沉默MUC16具有一定的抑制胆囊癌细胞增殖和迁移作用，可为胆囊癌的治疗提供新的实验证据。

       

      Abstract:
      ObjectiveTo explore the effects of siRNA silencing mucin 16(MUC16) on the proliferation and migration of gallbladder carcinoma GBC-SD cells, and its molecular mechanisms.
      MethodsThe GBC-SD+MUC16-siRNA group was constructed using the lentivirus infection and siRNA interference technique to silence MUC16 gene in GBC-SD cells, and the negative control group(GBC-SD+NC group) and blank control group(GBC-SD group) were set up simultaneously.The RT-PCR was used to detect the expression of MUC16 in GBC-SD cells, the MTT and scratch assays were used to detect the proliferation and migration ability of GBC-SD cells, and the Western blotting was used to detect the protein expressions of CD44 and PCNA in GBC-SD cells.
      ResultsThe cell proliferation rate and cell migration number in GBC-SD+MUC16-SiRNA group were lower than those in GBC-SD group(P < 0.05).The expression levels of MUC16 and CD44 protein in GBC-SD+MUC16-SIRNA group were lower than those in GBC-SD group and GBC-SD+NC group(P < 0.05 to P < 0.01), and the expression level of PCNA protein in GBC-SD+MUC16-SIRNA group was also lower than that in GBC-SD+NC group(P < 0.05).
      ConclusionsSiRNA silencing MUC16 can inhibit the proliferation and migration of gallbladder cancer cells, which may provide the new experimental evidence for the treatment of gallbladder cancer.

       

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