circ_0000515靶向miR-1258调控结肠癌细胞增殖和凋亡的机制研究

汪景坤; 樊丽伟; 李洵; 付晓霞; 赵彦焕; 张东姣; 赵玉红; 张磊

doi:10.13898/j.cnki.issn.1000-2200.2021.12.002

circ_0000515靶向miR-1258调控结肠癌细胞增殖和凋亡的机制研究

Effect of circ_0000515 targeting miR-1258 on the proliferation and apoptosis in the colon cancer cells

摘要

摘要:
目的探讨circ_0000515调控结肠癌细胞增殖和凋亡的分子机制。
方法选取41例结肠癌病人癌组织及癌旁组织标本，用实时荧光定量PCR（RT-qPCR）检测circ_0000515和miR-1258的表达水平；结肠癌细胞SW620分为si-circ_0000515组、si-NC组、miR-1258组、miR-NC组、si-circ_0000515+anti-miR-NC组、si-circ_0000515+anti-miR-1258组。四甲基偶氮唑盐比色法（MTT）检测SW620细胞活性；流式细胞术检测SW620细胞凋亡；双荧光素酶报告实验检测circ_0000515和miR-1258的靶向关系。
结果与癌旁组织比较，结肠癌组织中circ_0000515表达水平升高，miR-1258表达水平降低（P < 0.01）。抑制circ_0000515表达或过表达miR-1258，SW620细胞活性降低，SW620细胞的凋亡率升高（P < 0.01）。circ_0000515靶向调控miR-1258；干扰miR-1258表达逆转了抑制circ_0000515表达对结肠癌SW620细胞增殖和凋亡的作用。
结论抑制circ_0000515表达通过靶向上调miR-1258抑制结肠癌细胞增殖，促进凋亡。

Abstract:
ObjectiveTo explore the molecular mechanism of circ_0000515 regulating the proliferation and apoptosis of colon cancer cells.
MethodsA total of 41 cases of colon cancer patients with cancer tissue and adjacent tissue samples were selected.Real-time fluorescent quantitative PCR was used to detect the expression of circ_0000515 and miR-1258.Colon cancer cells SW620 were divided into si-circ_0000515 group, si-NC group, miR-1258 group, miR-NC group, si-circ_0000515+anti-miR-NC group, si-circ_0000515+anti-miR-1258 group.Tetramethylazolium salt colorimetric assay(MTT) was used to detect the cell viability.Flow cytometry was used to detect apoptosis.The targeting relationship of circ_0000515 and miR-1258 was detected by dual-luciferase reporter assay.
ResultsCompared with the adjacent tissues, the expression of circ_0000515 in colon cancer tissues was increased, and the expression of miR-1258 was decreased(P < 0.01).After the inhibition of circ_0000515 expression or overexpression of miR-1258, the viability of SW620 cells was decreased, the apoptosis rate was increased(P < 0.01).Circ_0000515 targeted and regulated the expression of miR-1258.Interference with miR-1258 expression reversed the effect of inhibiting circ_0000515 expression on the proliferation and apoptosis of colon cancer SW620 cells.
ConclusionsInhibiting the expression of circ_0000515 by up-regulation of miR-1258 can inhibit the proliferation and promote the apoptosis in colon cancer cells.

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