刘华长, 杨彦立, 薛增明, 任珊. miR-503-5p对IL-1β诱导的血管内皮细胞增殖、迁移、凋亡和黏附的影响[J]. 蚌埠医科大学学报, 2021, 46(12): 1668-1672. DOI: 10.13898/j.cnki.issn.1000-2200.2021.12.006
    引用本文: 刘华长, 杨彦立, 薛增明, 任珊. miR-503-5p对IL-1β诱导的血管内皮细胞增殖、迁移、凋亡和黏附的影响[J]. 蚌埠医科大学学报, 2021, 46(12): 1668-1672. DOI: 10.13898/j.cnki.issn.1000-2200.2021.12.006
    LIU Hua-chang, YANG Yan-li, XUE Zeng-ming, REN Shan. Effects of miR-503-5p on the proliferation, migration, apoptosis and adhesion of vascular endothelial cells induced by IL-1β[J]. Journal of Bengbu Medical University, 2021, 46(12): 1668-1672. DOI: 10.13898/j.cnki.issn.1000-2200.2021.12.006
    Citation: LIU Hua-chang, YANG Yan-li, XUE Zeng-ming, REN Shan. Effects of miR-503-5p on the proliferation, migration, apoptosis and adhesion of vascular endothelial cells induced by IL-1β[J]. Journal of Bengbu Medical University, 2021, 46(12): 1668-1672. DOI: 10.13898/j.cnki.issn.1000-2200.2021.12.006

    miR-503-5p对IL-1β诱导的血管内皮细胞增殖、迁移、凋亡和黏附的影响

    Effects of miR-503-5p on the proliferation, migration, apoptosis and adhesion of vascular endothelial cells induced by IL-1β

    • 摘要:
      目的探讨miR-503-5p对白细胞介素1β(IL-1β)诱导的血管内皮细胞增殖、迁移、凋亡和黏附的影响和分子机制。
      方法将人脐静脉血管内皮细胞(HUVECs)分为NC组、IL-1β组、IL-1β+anti-miR-con组及IL-1β+anti-miR-503-5p组。分别采用噻唑蓝(MTT)法、黏附实验、流式细胞术、Transwell实验检测HUVECs增殖、黏附、凋亡和迁移能力。Western blotting检测细胞间黏附分子1(ICAM-1)、血管细胞黏附分子1(VCAM-1)表达水平。
      结果与NC组比较,IL-1β组HUVECs细胞活力、迁移细胞数显著降低,细胞黏附数、凋亡率、ICAM-1和VCAM-1蛋白表达显著升高(P < 0.01)。与IL-1β+anti-miR-con组比较,IL-1β+anti-miR-503-5p组HUVECs细胞活力、迁移细胞数显著升高,细胞黏附数、凋亡率、ICAM-1和VCAM-1蛋白表达显著降低(P < 0.01)。
      结论抑制miR-503-5p表达可促进血管内皮细胞增殖和迁移,抑制细胞黏附和凋亡,其机制可能与抑制ICAM-1和VCAM-1表达有关。

       

      Abstract:
      ObjectiveTo explore the effects of miR-503-5p on the proliferation, migration, apoptosis and adhesion of human umbilical vein endothelial cells(HUVECs) induced by interleukin1β(IL-1β), and its molecular mechanism.
      MethodsThe HUVECs were divided into the NC group, IL-1β group, IL-1β+anti-miR-con group and IL-1β+anti-miR-503-5p group.The proliferation, adhesion, apoptosis and migration ability of cells were detected using MTT assay, adhesion assay, flow cytometry and Transwell assay, respectively.The expression levels of intercellular adhesion molecule 1(ICAM-1) and vascular cell adhesion molecule 1(VCAM-1) were detected using Western blotting.
      ResultsCompared with the NC group, the cell viability and migrated cell numbers of HUVECs significantly reduced, and the cell adhesion numbers, apoptosis rate, and ICAM-1 and VCAM-1 protein expression levels significantly increased in the IL-1β group(P < 0.01).Compared with the IL-1β+anti-miR-con group, the cell viability and migrated cell numbers of HUVECs significantly increased, and the cell adhesion numbers, apoptosis rate, and ICAM-1 and VCAM-1 protein expression levels significantly decreased in the IL-1β+anti-miR-503-5p group(P < 0.01).
      ConclusionsInhibiting the miR-503-5p expression can promote the vascular endothelial cell proliferation and migration, and inhibit vascular adhesion and apoptosis, which may be related to the inhibition of ICAM-1 and VCAM-1 expression.

       

    /

    返回文章
    返回