Abstract:
ObjectiveTo construct a Trib3 knockout rat model and identify the related genotype.
MethodsAfter targeted knockout of Trib3 gene in rat model, the deletion of exon 3 of Trib3 gene was detected, and the genotype was identified by gene sequencing and PCR methods.
ResultsThe positive F0 generation of Trib3-/+ rats was obtained, the F1 and F2 generations were obtained by self-crossing, and the F2 generation of Trib3-/- rats was selected.
ConclusionsTrib3 knockout rat models are successfully prepared, and Trib3-/-, Trib3-/+ and Trib3+/+ rats are successively identified and can be stably inherited, it provides a theoretical basis for subsequent experiments.