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    王艳秋. miR-20a靶向PTEN调控垂体腺瘤细胞的增殖与侵袭[J]. 蚌埠医科大学学报, 2022, 47(5): 561-565. DOI: 10.13898/j.cnki.issn.1000-2200.2022.05.001
    引用本文: 王艳秋. miR-20a靶向PTEN调控垂体腺瘤细胞的增殖与侵袭[J]. 蚌埠医科大学学报, 2022, 47(5): 561-565. DOI: 10.13898/j.cnki.issn.1000-2200.2022.05.001
    shu
    WANG Yan-qiu. Study on the miR-20a regulating the proliferation and invasion of pituitary adenoma cells by targeting PTEN[J]. Journal of Bengbu Medical University, 2022, 47(5): 561-565. DOI: 10.13898/j.cnki.issn.1000-2200.2022.05.001
    Citation: WANG Yan-qiu. Study on the miR-20a regulating the proliferation and invasion of pituitary adenoma cells by targeting PTEN[J]. Journal of Bengbu Medical University, 2022, 47(5): 561-565. DOI: 10.13898/j.cnki.issn.1000-2200.2022.05.001
    shu

    miR-20a靶向PTEN调控垂体腺瘤细胞的增殖与侵袭

    Study on the miR-20a regulating the proliferation and invasion of pituitary adenoma cells by targeting PTEN

      摘要
    • 摘要:
      目的探讨miR-20a对垂体腺瘤细胞增殖和侵袭的影响及其作用机制。
      方法培养垂体腺瘤细胞系GH3、HP75，采用RT-qPCR、Western blotting、双荧光素酶报告基因检测等方法，分析垂体腺瘤细胞系中miR-20a和人第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)的表达相关性以及相互作用情况；采用CCK8和Transwell等方法检测miR-20a对垂体腺瘤细胞增殖和侵袭能力的影响，进一步考察对PTEN下游PI3K/AKT信号通路的影响。
      结果 miR-20a负调控PTEN的表达。体外细胞实验显示，与miR-20a类似物对照组相比，类似物组在垂体腺瘤细胞系GH3和HP75中细胞迁移细胞数量、细胞增殖相关蛋白PI3K、AKT、MAPK和侵袭相关蛋白E-cadherin、MMP2和MMP9的表达量均增高，差异均有统计学意义(P < 0.01)；与抑制物对照组相比，miR-20a抑制物组在GH3和HP75中细胞迁移细胞数量和各蛋白表达量均下降，差异均有统计学意义(P < 0.01)；双荧光素酶报告基因分析发现与miR-20a类似物对照组比较，类似物组PTEN-WT的活性降低(P < 0.01)。
      结论miR-20a在调节细胞的增殖和侵袭的过程中扮演非常重要的作用，因而影响了垂体腺瘤细胞的增殖和侵袭。它可能通过靶向PTEN，影响PI3K/AKT信号通路，参与垂体腺瘤的进程中。

       

      Abstract:
      ObjectiveTo explore the effects of miR-20a on the proliferation and invasion of pituitary adenoma cells and its mechanism.
      MethodsThe pituitary adenoma cell lines GH3 and HP75 were cultured, and the expression correlation and interaction of miR-20a and gene of phosphate and tensin homolog deletedon chromosome ten(PTEN) in cell lines were analyzed by RT-QPCR, Western blotting and double luciferase reporter gene detection.The CCK8 and Transwell methods were used to detect the effects of miR-20a on the proliferation and invasion ability of pituitary adenoma cells, and the effects of miR-20a on the downstream PI3K/AKT signaling pathway of PTEN were further investigated.
      ResultsmiR-20a can target PTEN and negatively regulate the expression of PTEN.The results of in vitro cell experiments showed that, compared with miR-20a analogue control group, the number of migrating cells, the expression levels of proliferation-related proteins PI3K, AKT, MAPK and invasion-related proteins E-cadherin, MMP2 and MMP9 in the pituitary adenoma cell lines GH3 and HP75 in the analogue group were increased(P < 0.01).Compared with the inhibitor control group, the number of migrating cells and the expression levels of each protein in miR-20a inhibitor group were decreased in GH3 and HP75, and the differences were statistically significant(P < 0.01).Double luciferase reporter gene analysis showed that compared with miR-20a analogue control group, the activity of PTEN-WT in the analogue group was decreased(P < 0.01).
      ConclusionsThe miR-20a plays an important role in the regulation of cell proliferation and invasion, and affects the growth and invasion of pituitary adenoma cells.It may regulate the PI3K/AKT signaling pathway and participate in the disease progression of pituitary adenoma by regulating the expression of PTEN.

       

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