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    苏凯, 赵报, 马莹晔, 李慧, 周帅, 王晓敏, 张俊杰, 马士崟. Vγ9Vδ2 T细胞联合顺铂增强对鼻咽癌细胞的杀伤作用[J]. 蚌埠医科大学学报, 2022, 47(11): 1479-1483. DOI: 10.13898/j.cnki.issn.1000-2200.2022.11.001
    引用本文: 苏凯, 赵报, 马莹晔, 李慧, 周帅, 王晓敏, 张俊杰, 马士崟. Vγ9Vδ2 T细胞联合顺铂增强对鼻咽癌细胞的杀伤作用[J]. 蚌埠医科大学学报, 2022, 47(11): 1479-1483. DOI: 10.13898/j.cnki.issn.1000-2200.2022.11.001
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    SU Kai, ZHAO Bao, MA Ying-ye, LI Hui, ZHOU Shuai, WANG Xiao-min, ZHANG Jun-jie, MA Shi-yin. Vγ9Vδ2 T cells combined with on Cisplatin enhance the killing effect of nasopharyngeal carcinoma cells[J]. Journal of Bengbu Medical University, 2022, 47(11): 1479-1483. DOI: 10.13898/j.cnki.issn.1000-2200.2022.11.001
    Citation: SU Kai, ZHAO Bao, MA Ying-ye, LI Hui, ZHOU Shuai, WANG Xiao-min, ZHANG Jun-jie, MA Shi-yin. Vγ9Vδ2 T cells combined with on Cisplatin enhance the killing effect of nasopharyngeal carcinoma cells[J]. Journal of Bengbu Medical University, 2022, 47(11): 1479-1483. DOI: 10.13898/j.cnki.issn.1000-2200.2022.11.001
    shu

    Vγ9Vδ2 T细胞联合顺铂增强对鼻咽癌细胞的杀伤作用

    Vγ9Vδ2 T cells combined with on Cisplatin enhance the killing effect of nasopharyngeal carcinoma cells

      摘要
    • 摘要:
      目的探讨Vγ9Vδ2 T免疫细胞联合顺铂（CDDP）对鼻咽癌细胞的杀伤作用。
      方法采用CCK8法观察低浓度CDDP、唑来膦酸（ZOL）联合Vγ9Vδ2 T细胞对鼻咽癌细胞增殖活性的影响；流式细胞术检测Vγ9Vδ2 T细胞肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）和CD107a的分泌及鼻咽癌细胞表面MICA和MICB的表达；Western blotting检测MICA和MICB的蛋白表达。
      结果随着CDDP浓度的增加，鼻咽癌CNE2Z细胞存活率降低，CDDP联合Vγ9Vδ2 T细胞的杀伤作用优于单独使用CDDP对CNE2Z细胞的杀伤作用（P < 0.05）。ZOL对Vγ9Vδ2 T细胞的增殖有促进作用，随着ZOL浓度提高，ZOL联合Vγ9Vδ2 T细胞对CNE2Z细胞的杀伤作用增强（P < 0.05）。低浓度CDDP和ZOL联合Vγ9Vδ2 T细胞可增强对鼻咽癌细胞的杀伤作用，当效靶比为10:1时，联合处理的杀伤效果最好，差异有统计学意义（P < 0.05）。低浓度CDDP、ZOL处理的鼻咽癌细胞可增强Vγ9Vδ2 T细胞免疫因子CD107a、TNF-α和IFN-γ的分泌（P < 0.05）。低浓度CDDP联合Vγ9Vδ2 T细胞明显上调鼻咽癌细胞表面MICA和MICB的表达（P < 0.01）。
      结论CDDP能增强Vγ9Vδ2 T细胞对鼻咽癌细胞的杀伤作用，其机制可能与促进鼻咽癌细胞表面MICA和MICB的表达及增加Vγ9Vδ2 T细胞TNF-α和IFN-γ的分泌有关。

       

      Abstract:
      ObjectiveTo investigate the killing effect of Vγ9Vδ2 T immune cells combined with cisplatin(CDDP) on nasopharyngeal carcinoma cells.
      MethodsThe CCK8 method was used to observe the effect of low-concentration of CDDP and zoledronate(ZOL) combined with Vγ9Vδ2 T cells on the proliferation activity of nasopharyngeal carcinoma cells.Flow cytometry was applied to determine the secretion of tumor necrosis factor-α(TNF-α), interferon-γ(IFN-γ) and CD107a in Vγ9Vδ2 T cells, and the expression of MICA and MICB on the surface of nasopharyngeal carcinoma cells.Western blotting was employed to detect the protein expression of MICA and MICB in nasopharyngeal carcinoma cells.
      ResultsThe survival rate of nasopharyngeal carcinoma CNE2Z cells decreased with the increase of CDDP concentration, the killing effect of CDDP combined with Vγ9Vδ2 T cells on CNE2Z cells was better than that of CDDP alone(P < 0.05).ZOL promoted the proliferation of Vγ9Vδ2 T cells, and the killing effect of ZOL combined with Vγ9Vδ2 T cells on CNE2Z cells increased with the increase of ZOL concentration(P < 0.05).Low-concentration of CDDP and ZOL combined with Vγ9Vδ2 T cells enhanced the killing effect on nasopharyngeal carcinoma cells, the killing effect of combined treatment was the best at the effect-target ratio of 10:1, and the difference of which was statistically significant(P < 0.05).Nasopharyngeal carcinoma cells treated with low-concentration of CDDP and ZOL could increase the secretion of immune factors CD107a, TNF-α and IFN-γ in Vγ9Vδ2 T cells(P < 0.05).Low-concentration of CDDP combined with Vγ9Vδ2 T cells significantly up-regulated the expression of MICA and MICB on the surface of nasopharyngeal carcinoma cells(P < 0.01).
      ConclusionsCDDP can enhance the killing effect of Vγ9Vδ2 T cells on nasopharyngeal carcinoma cells, which may be related to the enhanced expression of MICA and MICB on the surface of nasopharyngeal carcinoma cells and the increased secretion of TNF-α and IFN-γ in Vγ9Vδ2 T cells.

       

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