ObjectiveTo explore the effect of circPTPRA on the proliferation and migration of rheumatoid arthritis fibroblast-like synoviocytes and its regulatory effect on miR-140-5p.

MethodsThe primary human normal fibroblast-like synoviocyte and rheumatoid arthritis fibroblast-like synoviocyte were isolated and cultured.The synovial fibroblasts of rheumatoid arthritis were divided into NC group(without transfection), si-NC group(transfected si-NC), si-circPTPRA group (transfected si-circPTPRA), miR-NC group (transfected miR-NC) and miR-140-5p group (transfected miR-140-5p mimics), anti-miR-NC+si-circPTPRA group (co-transfected anti-miR-NC and si-circPTPRA), anti-miR-140-5p+si-circPTPRA group (co-transfected anti-miR-140-5p and si-circPTPRA).The expression levels of circPTPRA and miR-140-5p were detected by qRT-PCR.The cell proliferation was detected by MTT test, and the cell migration was tested by Transwell chamber.The targeting relationship between circPTPRA and miR-140-5p was detected through the dual luciferase reporter gene experiment.

ResultsCompared with normal fibroblast-like synoviocytes, in rheumatoid arthritis fibroblast-like synoviocytes, the expression of circPTPRA was increased, while the expression of miR-140-5p was decreased (P < 0.01).Compared with the si-NC group, in the si-circPTPRA group, the cell viability was decreased, the number of migrating cells was decreased (P < 0.05).Compared with the miR-NC group, in the miR-140-5p group, the cell viability and the number of migrating cells were decreased (P < 0.01).CircPTPRA could target miR-140-5p.Compared with the anti-miR-NC+si-circPTPRA group, the cell viability was increased as well as the number of migrating cells in the anti-miR-140-5p+si-circPTPRA group (P < 0.01).

ConclusionsInterference with the expression of circPTPRA can inhibit the proliferation and migration of rheumatoid arthritis fibroblast-like synoviocyte by targeting the expression of miR-140-5p.