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    姚楠, 孙景秋, 郭冰沁, 武世伍. PIK3R1在人原发性肝细胞癌中的表达及其与肿瘤上皮间质转化关系[J]. 蚌埠医科大学学报, 2023, 48(5): 570-573. DOI: 10.13898/j.cnki.issn.1000-2200.2023.05.003
    引用本文: 姚楠, 孙景秋, 郭冰沁, 武世伍. PIK3R1在人原发性肝细胞癌中的表达及其与肿瘤上皮间质转化关系[J]. 蚌埠医科大学学报, 2023, 48(5): 570-573. DOI: 10.13898/j.cnki.issn.1000-2200.2023.05.003
    shu
    YAO Nan, SUN Jing-qiu, GUO Bing-qin, WU Shi-wu. Expression of PIK3R1 in human primary hepatocellular carcinoma and its correlation with epithelial mesenchymal transformation[J]. Journal of Bengbu Medical University, 2023, 48(5): 570-573. DOI: 10.13898/j.cnki.issn.1000-2200.2023.05.003
    Citation: YAO Nan, SUN Jing-qiu, GUO Bing-qin, WU Shi-wu. Expression of PIK3R1 in human primary hepatocellular carcinoma and its correlation with epithelial mesenchymal transformation[J]. Journal of Bengbu Medical University, 2023, 48(5): 570-573. DOI: 10.13898/j.cnki.issn.1000-2200.2023.05.003
    shu

    PIK3R1在人原发性肝细胞癌中的表达及其与肿瘤上皮间质转化关系

    Expression of PIK3R1 in human primary hepatocellular carcinoma and its correlation with epithelial mesenchymal transformation

      摘要
    • 摘要:
      目的探讨磷脂酰肌醇-3激酶调节亚单位1(PIK3R1)在人原发性肝细胞癌(HCC)中的表达水平及其与恶性肿瘤上皮间质转化的相关性。
      方法选取80例HCC病人癌组织及相应癌旁组织，通过免疫组织化学染色法检测HCC组织及相应癌旁组织中PIK3R1编码蛋白(PIK3R1/p85α)表达，并检测HCC组织中EMT相关蛋白转化生长因子β(TGF-β)、上皮性钙黏蛋白(E-cadherin)表达，评估其表达与HCC病人临床特征的相关性。
      结果PIK3R1/p85α在HCC组织中的阳性率为70.0%(56/80)，高于癌旁组织的17.5%(7/40)(P < 0.05)；不同组织分化程度HCC病人的PIK3R1/p85α、E-cadherin和TGF-β表达差异均有统计学意义(P < 0.05~P < 0.01)；HCC组织中PIK3R1/p85α与E-cadherin表达呈明显负相关关系(r=-0.323，P < 0.01)，与TGF-β表达呈正相关关系(r=0.247，P < 0.05)；无侵袭转移HCC病人和有侵袭转移病人的PIK3R1/p85α蛋白表达差异有统计学意义(P < 0.05)。
      结论PIK3R1在HCC组织中表达水平升高，高水平PIK3R1可能会促进肿瘤上皮间质转化的发生，并与HCC不良预后有关。

       

      Abstract:
      ObjectiveTo investigate the expression level of phosphatidylinositol-3 kinase regulatory subunit 1(PIK3R1) in human primary hepatocellular carcinoma(HCC) and its correlation with epithelial mesenchymal transformation of malignant tumor.
      MethodsThe expression levels of PIK3R1 coding protein(PIK3R1/p85α) in HCC tissues and corresponding adjacent tissues of 80 HCC patients were detected by immunohistochemical staining. The expression levels of EMT-related proteins transforming growth factor β(TGF-β) and epithelial cadherin(E-cadherin) in HCC tissue were detected, and the correlation of the expression with clinical characteristics of HCC patients was evaluated.
      ResultsThe positive rate of PIK3R1 expression in HCC tissue(70.0%) was significantly higher than that in corresponding adjacent tissue(17.5%)(P < 0.05). The differences of the expression of PIK3R1/p85α, E-cadherin and TGF-β in HCC patients with different levels of tissue differentiation were statistically significant(P < 0.05 to P < 0.01). The expression of PIK3R1/p85α was negatively correlated with E-cadherin expression(r=-0.323, P < 0.01), and positively correlated with TGF-β expression in HCC tissue(r=0.247, P < 0.05). The difference of the protein expression of PIK3R1/p85α between non-invasive metastatic and invasive metastatic HCC patients was statistically significant(P < 0.05).
      ConclusionsThe expression level of PIK3R1 in HCC tissue increases. The high level of PIK3R1 may promote the epithelial mesenchymal transformation of tumors, and be associated with the poor prognosis of HCC.

       

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