ObjectiveTo analyze the transcriptome and proteome of salivary gland tissue and saliva in experimental Sjöogren′s syndrome(ESS) mice for further exploring the pathogenesis of primary Sjogren′s syndrome(pSS).

MethodsThe ESS mice model was established, the salivary gland tissue and saliva were harvested, and the relevant mRNA and protein expressions in salivary gland tissue and saliva were detected using high-throughput transcriptome sequencing and proteomic analysis based on LC-MS/MS, respectively. The pathway enrichment analysis was used to identify the abnormal regulatory pathways, and the further association analysis of RNA and protein was conducted.

ResultsThe ESS model was successfully verified by pathological examination of salivary glands and saliva volume measurement, and 3 221 differentially expressed genes and 253 differentially expressed proteins were identified. The results of integrated analysis of transcriptomic and proteomic data showed that 61 proteins overlapped. The results of the enrichment pathways of abnormally regulated genes and proteins analysis showed that the renin-angiotensin system(RAS) and lysosome were significantly related to the apoptosis signaling pathways. The transcription and protein levels of AGT, Fn1, Klk1b26, Klk1, Klk1b5 and Klk1b3 were consistent regulatory trends, which might be a potential diagnostic biomarker of pSS.

ConclusionsThe pathogenesis of pSS may be related to the RAS, lysosome and apoptosis signaling pathway, and the AGT, Fn1 and K1ks may be the key molecules of the pathogenesis of pSS.