基于16S rRNA基因测序的黄褐斑病人肠道菌群差异研究

李琬晴; 蔡玲玲; 郭丽媛; 王莹; 刘思含; 计广; 张丰川

doi:10.13898/j.cnki.issn.1000-2200.2023.06.012

基于16S rRNA基因测序的黄褐斑病人肠道菌群差异研究

Difference of intestinal flora in chloasma patients based on 16S rRNA gene sequencing

摘要

摘要:
目的利用新一代16S rRNA测序技术比较黄褐斑病人与健康人的肠道菌群多样性和细菌表型差异。
方法纳入30例黄褐斑病人为研究组，以20名正常人作为健康对照，测序分析粪便样品中细菌群落的结构组成和表型特征。
结果研究组肠道微生物群落丰度、多样性相对降低。研究组拟杆菌门、拟杆菌纲、拟杆菌目、普雷沃菌科、拟杆菌科、普雷沃氏菌、拟杆菌属等丰度显著降低，而厚壁菌门、放射菌门、放线菌纲、乳杆菌目、双歧杆菌目、毛螺菌科、布劳特氏菌等升高，差异均有统计学意义(P < 0.05~P < 0.01)。
结论黄褐斑病人与健康人肠道菌群存在一定差异。

Abstract:
ObjectiveTo compare the intestinal flora diversity and bacterial phenotypes between chloasma patients and healthy people by a new generation 16S rRNA sequencing technology.
MethodsThirty patients with chloasma were recruited as study group, while 20 healthy individuals were recruited as control group.Structural composition and phenotypic characteristics of bacterial community were analyzed in stool samples with sequencing.
ResultsThe abundance and diversity of the intestinal flora community were relatively reduced in the study group.Compared with control group, the abundance of Bacteroidota, Bacteroidia, Bacteroidales, Prevotellaceae, Bacteroidaceae, Prevotella, and Bacteroides were lower, while Firmicutes, Actinobacteriota, Actinobacteria, Lactobacillales, Bifidobacteriales, Lachnospiraceae, and Blautia were higher in study group (P < 0.05 to P < 0.01).
ConclusionsThe intestinal flora differs between the patients with chloasma and healthy people.

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