糖尿病合并代谢综合征病人血清miR-126a、miR-130b水平及与骨质疏松的关系

赵丽娟; 智新力; 孙智; 杨华

doi:10.13898/j.cnki.issn.1000-2200.2023.09.013

糖尿病合并代谢综合征病人血清miR-126a、miR-130b水平及与骨质疏松的关系

Study on the relationship between the serum levels of miR-126a and miR-130b and osteoporosis in patients with diabetes mellitus complicated with MS

摘要

摘要:
目的探讨2型糖尿病合并代谢综合征(MS)病人血清miRNA-126a、miRNA-130b水平及其与骨质疏松的关系。
方法选取162例2型糖尿病病人为研究对象，其中72例单纯糖尿病病人为对照组，90例糖尿病合并MS病人为观察组，同时根据是否发生骨质疏松将2型糖尿病合并MS病人分为骨质疏松组(52例)与非骨质疏松组(38例)。实时定量聚合酶链反应法检测病人血清miR-126a、miR-130b相对表达量；检测各项实验室指标及骨质疏松症相关因子水平。Pearson法分析骨质疏松病人血清miR-126a、miR-130b表达水平与骨质疏松症相关因子的相关性；多因素logistic回归分析法分析影响骨质疏松发生的相关因素。
结果观察组血清miR-126a、miR-130b表达水平均高于对照组(P < 0.05)；骨质疏松组病人体质量指数(BMI)、血红蛋白(Hb)、骨钙素、骨密度(BMD)、胰岛素样生长因子-1(IGF-1)均低于非骨质疏松组(P < 0.05)，而总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、糖化血红蛋白(HbA1c)、血清Ⅰ型前胶原氨基端肽原(P1NP)及血清miR-126a、miR-130b表达水平均升高(P < 0.05)；miR-126a、miR-130b均与BMI、Hb、骨钙素、BMD、IGF-1呈明显负相关关系(P < 0.01)，而与TC、TG、HDL-C、HbAlc、PINP呈明显正相关关系(P < 0.01)；logistic分析显示BMI、Hb、IGF-1及miR-126a、miR-130b表达水平均为骨质疏松发生的影响因素(P < 0.05~P < 0.01)。
结论2型糖尿病合并MS病人血清miR-126a、miR-130b表达水平升高可促进骨质疏松发生，并可能作为预测骨质疏松发生的重要辅助指标。

Abstract:
ObjectiveTo investigate the serum levels of micro RNA-126a(miR-126a) and microRNA-130b(miR-130b) in patients with type 2 diabetes mellitus complicated with metabolic syndrome(MS), and their relationships with osteoporosis.
MethodsA total of 162 patients with type 2 diabetes mellitus were selected.Seventy-two patients with simple diabetes mellitus and 90 patients with diabetes mellitus complicated with MS were divided into the control group and observation group, respectively.The patients with type 2 diabetes mellitus complicated with MS were subdivided into the osteoporosis group(52 cases) and non-osteoporosis group(38 cases).The relative expression levels of serum miR-126a and miR-130b were detected using the real-time quantitative polymerase chain reaction.The laboratory indicators and osteoporosis-related factors levels were detected.The correlation between the expression levels of serum miR-126a and miR-130b and osteoporosis-related factors were analyzed using Pearson method.The multivariate logistic regression analysis was used to analyze the related factors affecting the occurrence of osteoporosis.
ResultsThe expression levels of serum miR-126a and miR-130b in observation group were significantly higher than those in control group(P < 0.05).The body mass index(BMI), hemoglobin(Hb), osteocalcin, bone mineral density(BMD) and insulin-like growth factor(IGF-1) in osteoporosis group were significantly lower than those in non-osteoporosis group(P < 0.05), and the serum levels of total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL-C), glycosylated hemoglobin(HbA1c), procollagen type Ⅰ amino terminal peptide(P1NP), miR-126a and miR-130b in osteoporosis group significantly increased(P < 0.05).The levels of miR-126a and miR-130b were negatively correlated with BMI, Hb, osteocalcin, BMD and IGF-1(P < 0.01), but positively correlated with TC, TG, HDL-C, HbAlc and PINP(P < 0.01).The results of logistic analysis showed that the BMI, Hb, IGF-1, miR-126a and miR-130b expression levels were the influencing factors of osteoporosis(P < 0.05 to P < 0.01).
ConclusionsThe expression levels of serum miR-126a and miR-130b increasing in patients with type 2 diabetes mellitus complicated with MS can promote the occurrence of osteoporosis, and whcih may be used as an important auxiliary index to predict the occurrence of osteoporosis.

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