Abstract: Objective: To evaluate the clinical effects of the microscopic excision guided by sodium fluorescein in recurrent glioma.Methods: Among 46 recurrent glioma patients treated with operation,the tumors in 21 cases were excised with microscopic excision after the range of tumor was identified using sodium fluorescein guidance(sodium fluorescein guidance group),and 25 cases were excised with conventional microscopic resection(traditional operation group).The ranges of tumor resection in two groups were identified using MRI enhanced scan after operation.Results: The yellow fluorescence in recurrent glioma of sodium fluorescein guidance group were found under fluorescence microscope,the pathological results showed that a lot of glioma cells located in yellow fluorescence area,and the postoperative MRI enhanced scan results showed that the tumor was thoroughly resected.The total resection rate in sodium fluorescein guidance group(91.7%) was higher than that in traditional operation group(65.3%)(P<0.05).The KPS score in fluoresce sodium guidance group(80.6±5.7 was higher than that in traditional operation group(75.6±6.3)(P<0.01).Conclusions: The microscopic excision guided by sodium fluorescein in recurrent glioma can accurately determine the tumor boundary,improve the rate of tumor resection rate and protect the normal brain tissue.