Abstract: Objective: To observe dynamic expression of NF-κB in pancreas tissues and cytokines in serum and to explore the correlation between NF-κB and cytokine in rats with acute pancreatitis.Methods: Fifty Sprayue-Dawley rats were randomly divided into normal control group,acute edematous pancreatitis group (AEP),3 h acute necrotizing pancreatitis(ANP 3 h)group,ANP 6 h group and ANP 12 h group.The AEP model was successfully made by injecting cerulein into the abdominal cavity of the rats; ANP was induced by intraductal administration of 5% sodium taurocholate.The serum amylase,interlukin-6 (IL-6),tumor necrosis factor-α (TNF-α),and the expression of NF-κB in pancreas tissues and pancreatic pathology were recorded.Results: The expression of TNF-α,IL-6 and NF-κB increased significantly in AEP group(P<0.01).The expression of TNF-α,IL-6 and NF-κB was more evident in ANP group than that in AEP group,which reached the peak at hour 6,then began to come down.Conclusions: NF-κB in pancreas tissues takes an important role in the pathogenesis of acute pancreatitis and the expression of NF-κB has a positive correlation with TNF-α,IL-6.