Abstract: Objective:To analyze the change trend of the pathogenic bacteria distribution and drug resistance in patients with urinary tract infection so as to provide basis for the rational use of drugs in clinic． Methods:The pathogenic bacteria distribution of 668 strains isolated from a hospital and the distribution and drug resistance of extended-spectrum -lactamase(ESBL) Escherichia coli(E． coli) were retrospectively analyzed from 2006 to 2011． Results:Six hundred and sixty-eight strains uropathogen ( the isolation rate for 28． 4%) were isolated from 2 356 urine specimens from 2006 to 2011，which included 438 strains Gram-negative bacilli(65． 6%)， 120 strains Gram-positive Cocci (18． 0%) and 110 stains Cadidia spp (16． 4%)． E． coli was the most commonest uropathogen (302/668)，followed by Candida albicans(57/668) and Klebsiella pneumonia(47/668)． The isolation rate of ESBL + E． coli was 54． 3%(164/302)，and the annual isolation rates of ESBL E． coli were 50． 0% (14/28)，51． 9% (28/54)，45． 6% (26/57)，45． 8% (22/48)，53． 1%(26/49) and 72． 7%(48/66) during the consecutive 6-year，respectively． The urinary tract infection in females(422 cases) were more than that in males(246 cases)，the females with age more than 50 years were the majority(419/668)． Conclusions: The E． coli is the major uropathogen in urinary tract infection，ESBLs is high islation rate and antibiotic resistance，and the islation rate of Candida albicans is higher．