鞣花酸对转染并稳定表达Cx32的HeLa细胞缝隙连接功能的影响

The effect of ellagic acid on the function of gap junction of Hela cells with stable expresson of Cx32

摘要: 目的：观察鞣花酸(EA)对转染并稳定表达Cx32的HeLa细胞中缝隙连接(GJ)通讯功能的影响。方法：细胞接种荧光示踪法观察EA对GJ功能的影响；细胞免疫荧光法检测EA对细胞膜上Cx32表达的影响。结果：EA 4、8、16 mol/L分别使细胞荧光渗透率显著增强6.04%、12.50%和20.33%(P0.01)，使细胞膜Cx32的荧光强度显著增强23.66%、46.09%和92.18%(P0.01)。结论：EA可通过增强胞膜Cx32的表达，增强细胞GJ通讯功能。

Abstract: Objective:To investigate the effects of ellagic acid(EA) on the intercellular communication of gap junction(GJ) in Hela cells with stable expresson of Cx32.Methods:The effects of EA on the GJ function were observed by cell labelling fluorescent tracer method.The effects of EA on the Cx32 expression on the membrane of Hela cells were detected by immunofluorescence assay.Results:The cells were treated with 4,8 and 16 mol/L of EA,the penetration rates of cell fluorescence increased by 6.04%,12.50% and 20.33%,respectively(P0.01),the fluorescence intensity of the Cx32 on the membrane increased by 23.66%,46.09% and 92.18%,respectively(P0.01).Conclusions:EA can enhance the communication function of GJ in Hela cells by promoting the Cx32 on the membrane expression.