肿瘤患者化疗前后血清IL-2、IL-18和TNF-α检测及临床意义

Detection and clinical significance of serum IL-2, IL-18 and TNF-α in tumor patients before and after chemotherapy

摘要: 目的: 探讨化疗药物对机体免疫系统是否存在不同程度的抑制作用。方法: 采用间接法亲和素-生物素复合物技术和酶联免疫吸附试验(ABC-ELISA)检测32例恶性实体肿瘤患者化疗一个周期前后细胞因子白细胞介素2(interleukin 2,IL-2)、IL-18、肿瘤坏死因子-α(TNF-α)水平。结果: IL-2、IL-18、TNF-α在肿瘤患者化疗前后变化均不显著(P>0.05)。结论: 机体的细胞免疫系统在化疗药物细胞毒性物质作用下能够发挥较强的自我调节能力,维持自身细胞免疫水平于一定的稳定状态,从而发挥其免疫功能。

Abstract: Objective: To investigate the influence of chemotherapy on the immune system.Methods: The serum IL-2,IL-18 and TNF-α in 32 patients with malignant solid tumor were measured by indirect avidin-biotin complex technique and enzyme-linked immunosorbent assay.Results: The experiment showed that the levels of IL-2,IL-18 and TNF-α did not have significant difference before and after chemotherapy one period(P>0.05).Conclusions: The cell-mediated immunity system has strong self-regulated ability when affected by cytotoxic substance in order to maintain its stability and perform its immunity function.