Abstract: Objective:To determine the formaldehyde concentration in the air of anatomy laboratories at medical colleges.Methods:Medium flow air samplers were used for sampling;high-performance liquid chromatography was adopted;acetonitrile solution with 2,4-dinitrophenyl hydrazine was used as the Absorption liquid,and aqueous acetonitrile as the mobile phase;C18 reversed-phase column was applied for the separation;ultraviolet and visible light purple outside-visible detector was measured at 365 nm wavelength.Results:According to the analysis results,the formaldehyde concentration in the air was as high as 2.8 mg/m³.Conclusions:The concentration of formaldehyde in the air of the anatomy laboratory in a certain medical college is 23.3 times higher than the national standard in public places.It is necessary to ameliorate the environment where teachers and students are working and studying.