Abstract: Objective: To establish a non-invasive diagnostic model for non-alcoholic fatty liver disease(NAFLD) based on sterol regulatory element-binding protein-1c(SREBP-1c), combined with white blood cell count(WBC), alanine aminotransferase(ALT) and triglyceride (TG), and clarify the efficacy and application value of SREBP-1c in the diagnosis of NAFLD. Methods: A total of 175 patients with NAFLD were selected as the research subjects, and 144 healthy individuals were selected as the healthy control group.The expression level of serum SREBP-1c was detected by double antibody sandwich enzyme-linked immunosorbent assay.The levels of indicators such as serum ALT and TG were detected by automatic biochemical analyzer, and the peripheral blood WBC was detected by automatic blood cell analyzer.The ROC curve was used to evaluate the efficacy of the above four indicators and non-invasive diagnostic model constructed by them in diagnosing NAFLD. Results: Compared with the healthy control group, the levels of SREBP-1c, WBC, ALT, TG, aspartate aminotransferase(AST), γ-glutamyl transferase(γ-GGT), and fasting plasma glucose(FPG) in the NAFLD group significantly increased(P < 0.05 to P < 0.01).The results of binary logistic analysis showed that the increasing of SREBP-1c, WBC, ALT and TG levels were the independent risk factors of NAFLD(OR=1.435, 1.042, 2.113, 1.012, P < 0.05 to P < 0.01).The area under the ROC curve of the clinical non-invasive diagnostic model established based on SREBP-1c in combination with WBC, ALT and TG was 0.918 2, the sensitivity was 84.03%, the specificity was 84.00%, and the diagnostic efficacy was higher than that of a single indicator. Conclusions: The clinical non-invasive diagnostic model based on SREBP-1c can effectively improve the accuracy of NAFLD diagnosis and provide a reference basis for clinical diagnosis and treatment.